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Enhanced dihydroflavonol-4-reductase activity and NAD homeostasis leading to cell death tolerance in transgenic rice

Hayashi, M ; Takahashi, H ; Tamura, K ; Huang, J ; Yu, L.H ; Kawai-Yamada, M ; Tezuka, T ; Uchimiya, H

Proceedings of the National Academy of Sciences - PNAS, 2005-05, Vol.102 (19), p.7020-7025 [Periódico revisado por pares]

United States: National Academy of Sciences

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Enhanced dihydroflavonol-4-reductase activity and NAD homeostasis leading to cell death tolerance in transgenic rice
Autor: Hayashi, M ; Takahashi, H ; Tamura, K ; Huang, J ; Yu, L.H ; Kawai-Yamada, M ; Tezuka, T ; Uchimiya, H
Assuntos: Alcohol Oxidoreductases - metabolism ; Amide Synthases - metabolism ; Bacterial diseases ; bacterial diseases of plants ; Biological Sciences ; Cell Death ; Cochliobolus carbonum ; Coenzymes ; Coloring Agents - pharmacology ; Corn ; Dehydrogenases ; dihydroflavonol-4-reductase ; disease resistance ; Electrophoresis, Capillary ; enzyme activity ; Enzymes ; Evans Blue - pharmacology ; gene overexpression ; Glutathione Transferase - metabolism ; Homeostasis ; host-pathogen relationships ; hydrogen peroxide ; Hydrogen-Ion Concentration ; Ions ; Mass Spectrometry ; Models, Biological ; NAD (coenzyme) ; NAD - chemistry ; NAD - metabolism ; NADP (coenzyme) ; NADP - chemistry ; Niacinamide - chemistry ; Oryza - genetics ; Oryza sativa ; oxidoreductases ; Pathogens ; Phosphotransferases (Alcohol Group Acceptor) - metabolism ; Plant Leaves - metabolism ; plant pathogenic bacteria ; Plants, Genetically Modified ; Reactive Oxygen Species ; Recombinant Fusion Proteins - metabolism ; Rice ; Seedlings ; stress tolerance ; Time Factors ; Transgenic plants ; Xanthomonas oryzae pv. oryzae ; Zea mays
É parte de: Proceedings of the National Academy of Sciences - PNAS, 2005-05, Vol.102 (19), p.7020-7025
Notas: ObjectType-Article-2
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Communicated by Takayoshi Higuchi, Kyoto University, Kyoto, Japan, March 28, 2005
Abbreviations: CE, capillary electrophoresis; DFR, dihydroflavonol-4-reductase; HCTR, NADPH-dependent HC-toxin reductase; NMNAT, ATP-NMN adenylyltransferase.
Author contributions: K.T., M.K.-Y., T.T., and H.U. designed research; M.H., H.T., K.T., J.H., and L.-H.Y. performed research; M.H., H.T., K.T., and H.U. analyzed data; and M.H., H.T., K.T., and H.U. wrote the paper.
To whom correspondence should be addressed. E-mail: uchimiya@iam.u-tokyo.ac.jp.
Descrição: The maize Hm1 gene encoding the NADPH-dependent HC-toxin reductase is capable of detoxifying HC-toxin of fungus Cochliobolus carbonum. Here, we conducted the metabolic and biochemical analysis in transgenic rice plants overexpressing an HC-toxin reductase-like gene in rice (YK1 gene). Methods employing NADPH oxidation and capillary electrophoresis mass spectrometry analysis confirmed that YK1 possessed dihydroflavonol-4-reductase activity in vitro and in vivo. The overexpression of YK1 in both suspension-cultured cells and rice plants increased NAD(H) and NADP(H) levels by causing an increase in NAD synthetase and NAD kinase activities. Activity changes in enzymes that require NAD(P) as coenzymes were also noted in rice cells ectopically expressing YK1, where the cell death caused by hydrogen peroxide and bacterial disease was down-regulated. Thus, a strategy was proposed that the combination of dihydroflavonol-4-reductase activity and the elevated level of NAD(P)H pool may confer the prevention of induced cell death in planta.
Editor: United States: National Academy of Sciences
Idioma: Inglês

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Enhanced dihydroflavonol-4-reductase activity and NAD homeostasis leading to cell death tolerance in transgenic rice

Hayashi, M ; Takahashi, H ; Tamura, K ; Huang, J ; Yu, L.H ; Kawai-Yamada, M ; Tezuka, T ; Uchimiya, H

United States: National Academy of Sciences

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