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Activation-Induced Cytidine Deaminase Shuttles between Nucleus and Cytoplasm like Apolipoprotein B mRNA Editing Catalytic Polypeptide 1

Ito, Satomi ; Nagaoka, Hitoshi ; Shinkura, Reiko ; Begum, Nasim ; Muramatsu, Masamichi ; Nakata, Mikiyo ; Honjo, Tasuku

Proceedings of the National Academy of Sciences - PNAS, 2004-02, Vol.101 (7), p.1975-1980 [Periódico revisado por pares]

United States: National Academy of Sciences

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Título:
Activation-Induced Cytidine Deaminase Shuttles between Nucleus and Cytoplasm like Apolipoprotein B mRNA Editing Catalytic Polypeptide 1
Autor: Ito, Satomi ; Nagaoka, Hitoshi ; Shinkura, Reiko ; Begum, Nasim ; Muramatsu, Masamichi ; Nakata, Mikiyo ; Honjo, Tasuku
Assuntos: 3T3 cells ; AIDS ; Amino Acid Sequence ; Amino acids ; Animals ; APOBEC-1 Deaminase ; apolipoprotein B ; B lymphocytes ; B-Lymphocytes - cytology ; B-Lymphocytes - metabolism ; Biological Sciences ; Cell lines ; Cell Nucleus - metabolism ; Cells, Cultured ; Cytidine Deaminase - chemistry ; Cytidine Deaminase - genetics ; Cytidine Deaminase - metabolism ; Cytoplasm ; Cytoplasm - metabolism ; DNA ; Enzymes ; Humans ; Immunology ; Messenger RNA ; Mice ; Molecular Sequence Data ; Mutation ; NIH 3T3 Cells ; Nuclear Localization Signals ; Peptides ; Protein Structure, Tertiary ; Protein Transport ; Proteins ; Ribonucleic acid ; RNA ; RNA editing
É parte de: Proceedings of the National Academy of Sciences - PNAS, 2004-02, Vol.101 (7), p.1975-1980
Notas: ObjectType-Article-2
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To whom correspondence should be addressed. E-mail: honjo@mfour.med.kyoto-u.ac.jp.
Contributed by Tasuku Honjo, December 29, 2003
Abbreviations: AID, activation-induced cytidine deaminase; wt, wild type; SHM, somatic hypermutation; CSR, class switch recombination; APOBEC1, apolipoprotein B mRNA editing catalytic polypeptide 1; GC, gene conversion; NES, nuclear export signal; NLS, nuclear localization signal; ACF, APOBEC1 complementation factor; hAID, human AID; LMB, leptomycin B; PI, propidium iodide; tet, tetracycline.
Descrição: Activation-induced cytidine deaminase (AID) is a molecule central to initiating class switch recombination, somatic hypermutation, and gene conversion of Ig genes. However, its mechanism to initiate these genetic alterations is still unclear. AID can convert cytosine to uracil on either mRNA or DNA and is involved in DNA cleavage. Although these events are expected to take place in the nucleus, overexpressed AID was found predominantly in the cytoplasm. Here, we demonstrated that AID is a nucleocytoplasmic shuttling protein with a bipartite nuclear localization signal and a nuclear export signal in its N and C termini, respectively. In addition to previously identified genetic, structural, and biochemical similarities of AID with apolipoprotein B mRNA editing catalytic polypeptide 1, an RNA editing enzyme of ApoB100 mRNA, the present finding provides another aspect to their resemblance, suggesting that both may have homologous reaction mechanisms.
Editor: United States: National Academy of Sciences
Idioma: Inglês

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Activation-Induced Cytidine Deaminase Shuttles between Nucleus and Cytoplasm like Apolipoprotein B mRNA Editing Catalytic Polypeptide 1

Ito, Satomi ; Nagaoka, Hitoshi ; Shinkura, Reiko ; Begum, Nasim ; Muramatsu, Masamichi ; Nakata, Mikiyo ; Honjo, Tasuku

United States: National Academy of Sciences

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