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Is FAS/Fas Ligand System Involved in Equine Corpus Luteum Functional Regression?

Galvao, Antonio M ; Ramilo, David W ; Skarzynski, Dariusz J ; Lukasik, Karolina ; Tramontano, Angela ; Mollo, Antonio ; Mateus, Luisa M ; Ferreira-Dias, Graca Maria L

Biology of reproduction, 2010-12, Vol.83 (6), p.901-908 [Periódico revisado por pares]

Madison, WI: Society for the Study of Reproduction, Inc

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Título:
Is FAS/Fas Ligand System Involved in Equine Corpus Luteum Functional Regression?
Autor: Galvao, Antonio M ; Ramilo, David W ; Skarzynski, Dariusz J ; Lukasik, Karolina ; Tramontano, Angela ; Mollo, Antonio ; Mateus, Luisa M ; Ferreira-Dias, Graca Maria L
Assuntos: Animals ; Apoptosis ; Biological and medical sciences ; Cell Survival ; Cells, Cultured ; Corpus Luteum - cytology ; Corpus Luteum - metabolism ; Dinoprost - metabolism ; Dinoprostone - metabolism ; Fas Ligand Protein - genetics ; Fas Ligand Protein - metabolism ; fas Receptor - genetics ; fas Receptor - metabolism ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; Horses ; Immunohistochemistry ; Interferon-gamma - metabolism ; Luteal Phase - metabolism ; Luteolysis - metabolism ; Progesterone - metabolism ; RNA, Messenger - metabolism ; Signal Transduction ; Tumor Necrosis Factor-alpha - metabolism ; Vertebrates: reproduction
É parte de: Biology of reproduction, 2010-12, Vol.83 (6), p.901-908
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Proapoptotic factor Fas ligand (FASL) and its cell surface receptor FAS are tumor necrosis factor superfamily members that trigger apoptosis in different cell types. However, their influence on luteal steroidogenesis is not clearly understood. The aim of the present work was to determine (i) the presence of the cytokine FASL and its receptor FAS in the mare's corpus luteum (CL) throughout the luteal phase, as well as (ii) the influence of FASL alone, or together with the cytokines tumor necrosis factor alpha (TNF) and interferon gamma (IFNG), on equine luteal cell production of luteotrophic and luteolytic factors, cell viability, and apoptosis. FASL and FAS protein expression and mRNA transcription were evaluated in different luteal stages of the equine CL by Western blotting and real-time PCR assays, respectively. Protein expression and FASL mRNA transcription increased in the late CL. Also, FAS and FASL proteins were present in large steroidogenic and endothelial CL cells throughout the luteal phase, as demonstrated by immunohistochemistry. Equine luteal cells isolated from midluteal phase CL were stimulated without (control) or with exogenous cytokines: FASL (10 ng/ml); TNF+IFNG (10 ng/ml each; positive control) or FASL+TNF+IFNG (10 ng/ml each). FASL clearly inhibited in vitro progesterone and prostaglandin E₂ (PGE₂) production by equine luteal cells but increased prostaglandin F₂alpha (PGF₂alpha). Furthermore, FASL effect on equine luteal cell viability depended on the presence of cytokines TNF and IFNG. In conclusion, this study shows the presence of FASL and FAS in the equine CL and suggests their importance in functional luteolysis.
Editor: Madison, WI: Society for the Study of Reproduction, Inc
Idioma: Inglês

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Is FAS/Fas Ligand System Involved in Equine Corpus Luteum Functional Regression?

Galvao, Antonio M ; Ramilo, David W ; Skarzynski, Dariusz J ; Lukasik, Karolina ; Tramontano, Angela ; Mollo, Antonio ; Mateus, Luisa M ; Ferreira-Dias, Graca Maria L

Madison, WI: Society for the Study of Reproduction, Inc

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