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Resistence of rotaviruses adsorbed to zeta plus 60s filter membranes

P. Garrafa V Pauli; Dolores Ursula Mehnert; M J Vieira; Charlotte Marianna Hársi; Congresso do Instituto de Ciências Biomédicas (3. 2001 São Paulo)

Resumos São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2001

São Paulo Comissão de Cultura e Extensão Universitária do ICB/USP 2001

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  • Título:
    Resistence of rotaviruses adsorbed to zeta plus 60s filter membranes
  • Autor: P. Garrafa
  • V Pauli; Dolores Ursula Mehnert; M J Vieira; Charlotte Marianna Hársi; Congresso do Instituto de Ciências Biomédicas (3. 2001 São Paulo)
  • Assuntos: MICROBIOLOGIA
  • É parte de: Resumos São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2001
  • Descrição: A two-step virus concentration method has been routinely used in our lab for rotavirus detection in raw sewage and surface water samples. The application of such method for monitoring viruses in environmental samples is relatively simply. The first concentration step, based on filtration through electropositive filter membrane, can be done in loco, but the reconcentration one is restricted to a few laboratories due to the use of a ultracentrifuge. To enabling the use of this method in most of the brazilian counties, the filter membrane should be mailed by post to the nearest reference laboratory for elution and reconcentration of viruses. In order to evaluate for how long the rotaviruses resist adsorbed to a microporous filter membrane without significant loses of their infectivity, simian rotaviruses (104 FFU/50ml) were experimentaly seeded in one-liter of creek water and recovered by the filtration through Zeta Plus 60S filter membrane. The membranes were wrapped on plastic and stored at 4oC. The adsorbed viruses were eluted with beef-extract 3%-glicyne 0.05 M pH 9.0 after 6, 18, 24 and 48 h. Rotaviruses eluted immediately after filtration (t0) were considered as positive controls. Results show that rotaviruses can be recovered from positively charged membranes after 48h without significant loses of infectivity so that the membranes used for virus concentration can be mailed by post within this period
  • Editor: São Paulo Comissão de Cultura e Extensão Universitária do ICB/USP
  • Data de criação/publicação: 2001
  • Formato: 1 v. pôster 243.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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