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Manipulating the mouse embryo a laboratory manual

Brigid Hogan

Plainview, N.Y. Cold Spring Harbor Laboratory Press 1994

Localização: FMRP - Fac. Medicina de Ribeirão Preto    (616-092.9 H714m2 )(Acessar)

  • Título:
    Manipulating the mouse embryo a laboratory manual
  • Autor: Brigid Hogan
  • Assuntos: Mice -- Genetic engineering -- Laboratory manuals; Transgenic mice -- Laboratory manuals; Mice as laboratory animals; ANIMAIS DE LABORATÓRIO; Mice -- embryology -- laboratory manuals; Genetic Engineering -- laboratory manuals; Mice, Transgenic -- genetics -- laboratory manuals
  • Notas: Includes bibliographical references (p. 439-470) and index
  • Descrição: Developmental genetics and embryology of the mouse: past, present and future -- Summary of mouse development -- Setting up a colony for the production of transgenic and mutant mice -- Recovery, culture and transfer of embryos a nd germ cells -- Invitro manipulation of preimplantation embryos -- Production of transgenic mice -- Isolation, culture and manipulation of embryonic stem cells -- Analysis of transgenic mice -- Techniques for visualizing genes , gene products and specialized celltypes
    In vitro culture of eggs, embryos, primordial germ cells and teratocarcinoma cells -- Buffers and solutions -- Acidic tyrode solution for removing zonae -- Alkaline phosphatase buffers -- Alkaline phosphatase staining solution -- Avertin,anesthetic -- Bovine serum albumin (BSA) -- Cap++s/Mgp++s-free PBS -- Cap++s/Mgp++s-free tyrode ringer's saline (pH 7.6-7.7) -- Hyaluronidase -- NTM buffer (NTMT without tween 20) for in situ hybridization of intact embryos with RNAprobes -- NTMT buffer for in situ hybridization of intact embryos with RNA probes Pancreatin/trypsin solution for separating germ and tissue layers -- PBSMT for immunohistochemistry of whole mount embryos -- PBT -- Phenol/chloroform solution for isolating total RNA from mouse embryos or fetal tissues -- Phos phate-bufferedsaline (PBSö -- Pronase solution -- Saline/EDTA buffer plus glucose for isolation of germ cells and tissue culture -- 20X SSC -- 20X SSPE -- TE (tris/EDTA) buffer -- 0.25% trypsin in tris-saline for tissue culture -- Trypsin/EDTA solutions --Sources of information -- Genetic resources -- Books -- Journals Databases -- Software -- Suppliers -- Inbred, hybrid and random-bred mice -- Mouse genomic DNAs and RNAs -- Mouse genomic and cDNA libraries and clones -- Equipment and supplies
  • Editor: Plainview, N.Y. Cold Spring Harbor Laboratory Press
  • Data de criação/publicação: 1994
  • Formato: xvii, 497 p. ill. (some col.) 29 cm.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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