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Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum

Laursen, Tomas ; Borch, Jonas ; Knudsen, Camilla ; Bavishi, Krutika ; Torta, Federico ; Martens, Helle Juel ; Silvestro, Daniele ; Hatzakis, Nikos S. ; Wenk, Markus R. ; Dafforn, Timothy R. ; Olsen, Carl Erik ; Motawia, Mohammed Saddik ; Hamberger, Björn ; Møller, Birger Lindberg ; Bassard, Jean-Etienne

Science (American Association for the Advancement of Science), 2016-11, Vol.354 (6314), p.890-893 [Periódico revisado por pares]

United States: American Association for the Advancement of Science

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Título:
Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum
Autor: Laursen, Tomas ; Borch, Jonas ; Knudsen, Camilla ; Bavishi, Krutika ; Torta, Federico ; Martens, Helle Juel ; Silvestro, Daniele ; Hatzakis, Nikos S. ; Wenk, Markus R. ; Dafforn, Timothy R. ; Olsen, Carl Erik ; Motawia, Mohammed Saddik ; Hamberger, Björn ; Møller, Birger Lindberg ; Bassard, Jean-Etienne
Assuntos: Assembly ; Biocatalysis ; Biosynthetic Pathways ; Channeling ; Detergents - chemistry ; Dynamics ; Enzymes ; Fluorescence ; Genotype & phenotype ; Glucosyltransferases - chemistry ; Glucosyltransferases - isolation & purification ; Glucosyltransferases - metabolism ; Life Sciences ; Lipids - chemistry ; Lipids - isolation & purification ; Liposomes - chemistry ; Liposomes - metabolism ; Luminescent Proteins - analysis ; Luminescent Proteins - chemistry ; Metabolic syndrome ; Metabolites ; Multienzyme Complexes - chemistry ; Multienzyme Complexes - isolation & purification ; Multienzyme Complexes - metabolism ; Nitriles - metabolism ; Optical Imaging ; Plant Proteins - chemistry ; Plant Proteins - isolation & purification ; Plant Proteins - metabolism ; Plants (organisms) ; Protein Interaction Maps ; Red Fluorescent Protein ; Sorghum ; Sorghum - enzymology ; Spectrometry, Fluorescence ; Vegetal Biology
É parte de: Science (American Association for the Advancement of Science), 2016-11, Vol.354 (6314), p.890-893
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Metabolic highways may be orchestrated by the assembly of sequential enzymes into protein complexes, or metabolons, to facilitate efficient channeling of intermediates and to prevent undesired metabolic cross-talk while maintaining metabolic flexibility. Here we report the isolation of the dynamic metabolon that catalyzes the formation of the cyanogenic glucoside dhurrin, a defense compound produced in sorghum plants. The metabolon was reconstituted in liposomes, which demonstrated the importance of membrane surface charge and the presence of the glucosyltransferase for metabolic channeling. We used in planta fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy to study functional and structural characteristics of the metabolon. Understanding the regulation of biosynthetic metabolons offers opportunities to optimize synthetic biology approaches for efficient production of high-value products in heterologous hosts.
Editor: United States: American Association for the Advancement of Science
Idioma: Inglês

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Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum

United States: American Association for the Advancement of Science

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