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Bioactivity-Guided Fraction from Viscera of Abalone, Haliotis discus hannai Suppresses Cellular Basophils Activation and Anaphylaxis in Mice

Kap Seong Choi ; Tai-sun Shin ; Ginnae Ahn ; Shin Hye Kim ; Jiyeon Chun ; Mina Lee ; Dae Heon Kim ; Han-gil Choi ; Kyung-dong Lee ; Sun-yup Shim

Journal of microbiology and biotechnology, 2024-02, Vol.34 (2), p.379 [Periódico revisado por pares]

한국미생물생명공학회

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  • Título:
    Bioactivity-Guided Fraction from Viscera of Abalone, Haliotis discus hannai Suppresses Cellular Basophils Activation and Anaphylaxis in Mice
  • Autor: Kap Seong Choi ; Tai-sun Shin ; Ginnae Ahn ; Shin Hye Kim ; Jiyeon Chun ; Mina Lee ; Dae Heon Kim ; Han-gil Choi ; Kyung-dong Lee ; Sun-yup Shim
  • Assuntos: Abalone male viscera ; anaphylaxis ; basophils ; cytokine ; NF-κB
  • É parte de: Journal of microbiology and biotechnology, 2024-02, Vol.34 (2), p.379
  • Notas: The Korean Society for Applied Microbiology
  • Descrição: Basophils and mast cells are specialized effector cells in allergic reactions. Haliotis discus hannai (abalone), is valuable seafood. Abalone male viscera, which has a brownish color and has not been previously reported to show anti-allergic activities, was extracted with acetone. Six different acetone/hexane fractions (0, 10, 20, 30, 40, and 100%) were obtained using a silica column via β-hexosaminidase release inhibitory activity-guided selection in phorbol myristate acetate and a calcium ionophore, A23187 (PMACI)-induced human basophils, KU812F cells. The 40% acetone/hexane fraction (A40) exhibited the strongest inhibition of PMACI-induced-β-hexosaminidase release. This fraction dose-dependently inhibited reactive oxygen species (ROS) production and calcium mobilization without cytotoxicity. Western blot analysis revealed that A40 down-regulated PMACI-induced MAPK (ERK 1/2, p-38, and JNK) phosphorylation, and the NF-κB translocation from the cytosol to membrane. Moreover, A40 inhibited PMACI-induced interleukin (IL)-1β, IL-6, and IL-8 production. Anti-allergic activities of A40 were confirmed based on inhibitory effects on IL-4 and tumor necrosis factor alpha (TNF-α) production in compound (com) 48/80-induced rat basophilic leukemia (RBL)-2H3 cells. A40 inhibited β-hexosaminidase release and cytokine production such as IL-4 and TNF-α produced by com 48/80-stimulated RBL-2H3 cells. Furthermore, it’s fraction attenuated the IgE/DNP-induced passive cutaneous anaphylaxis (PCA) reaction in the ears of BALB/c mice. Our results suggest that abalone contains the active fraction, A40 is a potent therapeutic and functional material to treat allergic diseases.
  • Editor: 한국미생물생명공학회
  • Idioma: Coreano
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