Idioma:

Adrenaline in pro-oxidant conditions elicits intracellular survival pathways in isolated rat cardiomyocytes

Costa, Vera Marisa ; Silva, Renata ; Ferreira, Rita ; Amado, Francisco ; Carvalho, Félix ; de Lourdes Bastos, Maria ; Carvalho, Rui Albuquerque ; Carvalho, Márcia ; Remião, Fernando

Toxicology (Amsterdam), 2009-03, Vol.257 (1), p.70-79 [Periódico revisado por pares]

Kidlington: Elsevier Ireland Ltd

Texto completo disponível

Citações Citado por

Enviar para

Título:
Adrenaline in pro-oxidant conditions elicits intracellular survival pathways in isolated rat cardiomyocytes
Autor: Costa, Vera Marisa ; Silva, Renata ; Ferreira, Rita ; Amado, Francisco ; Carvalho, Félix ; de Lourdes Bastos, Maria ; Carvalho, Rui Albuquerque ; Carvalho, Márcia ; Remião, Fernando
Assuntos: 1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt - pharmacology ; Active Transport, Cell Nucleus ; Adrenaline ; Animals ; Biological and medical sciences ; Cardiomyocytes ; Caspase 3 - metabolism ; Cell Survival ; Cysteine Proteinase Inhibitors - pharmacology ; Cytochromes c - metabolism ; DNA-Binding Proteins - metabolism ; Emergency ; Epinephrine - metabolism ; Free Radical Scavengers - pharmacology ; Heart ; Heat shock factor-1 ; Heat shock factors ; Heat shock proteins ; Heat Shock Transcription Factors ; HSP27 Heat-Shock Proteins - metabolism ; HSP70 Heat-Shock Proteins - metabolism ; In Vitro Techniques ; L-Lactate Dehydrogenase - metabolism ; Leupeptins - pharmacology ; Male ; Medical sciences ; Mitochondria, Heart - metabolism ; Myocytes, Cardiac - drug effects ; Myocytes, Cardiac - metabolism ; Myocytes, Cardiac - pathology ; NF-kappa B - metabolism ; Nuclear factor-κB ; Oxidation-Reduction ; Oxidative stress ; Oxidative Stress - drug effects ; Proteasome Endopeptidase Complex - metabolism ; Proteasome Inhibitors ; Rats ; Reactive Oxygen Species - metabolism ; Signal Transduction - drug effects ; Time Factors ; Toxicology ; Transcription Factors - metabolism ; Xanthine - metabolism ; Xanthine Oxidase - metabolism
É parte de: Toxicology (Amsterdam), 2009-03, Vol.257 (1), p.70-79
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Abstract In several pathologic conditions, like cardiac ischemia/reperfusion, the sustained elevation of plasma and interstitial catecholamine levels, namely adrenaline (ADR), and the generation of reactive oxygen species (ROS) are hallmarks. The present work aimed to investigate in cardiomyocytes which intracellular signalling pathways are altered by ADR redox ability. To mimic pathologic conditions, freshly isolated calcium tolerant cardiomyocytes from adult rat were incubated with ADR alone or in the presence of a system capable of generating ROS [(xanthine with xanthine oxidase) (X/XO)]. ADR elicited a pro-oxidant signal with generation of reactive species, which was largely magnified by the ROS generating system. However, no change in cardiomyocytes viability was observed. The pro-oxidant signal promoted the translocation to the nucleus of the transcription factors, Heat shock factor-1 (HSF-1) and Nuclear factor-κB (NF-κB). In addition, proteasome activity was compromised in the experimental groups where the generation of reactive species occurred. The decrease in the proteasome activity of the ADR group resulted from its redox sensitivity, since the activity was recovered by adding the ROS scavenger, tiron. Proteasome inhibition seemed to elicit an increase in HSP70 levels. Furthermore, retention of mitochondrial cytochrome c and inhibition of caspase 3 activity were observed by X/XO incubation in presence or absence of ADR. In conclusion, in spite of all the insults inflicted to the cardiomyocytes, they were capable to activate intracellular responses that enabled their survival. These mechanisms, namely the pathways altered by catecholamine proteasome inhibition, should be further characterized, as they could be of relevance in the ischemia preconditioning and the reperfusion injury.
Editor: Kidlington: Elsevier Ireland Ltd
Idioma: Inglês

Links

Voltar para lista de resultados

Anterior Resultado 11 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Adrenaline in pro-oxidant conditions elicits intracellular survival pathways in isolated rat cardiomyocytes

Costa, Vera Marisa ; Silva, Renata ; Ferreira, Rita ; Amado, Francisco ; Carvalho, Félix ; de Lourdes Bastos, Maria ; Carvalho, Rui Albuquerque ; Carvalho, Márcia ; Remião, Fernando

Kidlington: Elsevier Ireland Ltd

Buscando em bases de dados remotas. Favor aguardar.