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Lipid–Lipid Interactions in Aminated Reduced Graphene Oxide Interface for Biosensing Application

Ali, Md. Azahar ; Kamil Reza, K ; Srivastava, Saurabh ; Agrawal, Ved Varun ; John, Renu ; Malhotra, Bansi Dhar

Langmuir, 2014-04, Vol.30 (14), p.4192-4201 [Periódico revisado por pares]

United States: American Chemical Society

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  • Título:
    Lipid–Lipid Interactions in Aminated Reduced Graphene Oxide Interface for Biosensing Application
  • Autor: Ali, Md. Azahar ; Kamil Reza, K ; Srivastava, Saurabh ; Agrawal, Ved Varun ; John, Renu ; Malhotra, Bansi Dhar
  • Assuntos: Amination ; Biosensing Techniques ; Electrochemical Techniques ; Electrodes ; Graphite - chemistry ; Lipids - analysis ; Oxidation-Reduction ; Oxides - chemistry ; Surface Properties
  • É parte de: Langmuir, 2014-04, Vol.30 (14), p.4192-4201
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: A label-free biosensor based on antiapolipoprotein B 100 functionalized-aminated reduced graphene oxide interface has been fabricated for detection of low density lipoprotein (LDL or lipid) cholesterol. The aminated reduced graphene oxide (NH2-rGO) based electrode surface is covalently functionalized with antiapolipoprotein B 100 (AAB or lipid) using EDC/NHS coupling chemistry. The lipid–lipid interactions at the NH2-rGO electrode surface have been investigated using electrochemical impedance spectroscopic technique. The structural and morphological investigations of NH2-rGO based immunosensor have been accomplished via transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, UV–visible, and electrochemical techniques. The impedimetric response of the proposed immunosensor shows excellent sensitivity (612 Ω mg–1 dL cm–2), a response time of 250 s, and a low detection limit of 5 mg/dL of LDL molecules. The association, dissociation, and equilibrium rate constants for this immunoelectrode are found to be 1.66 M–1 s–1, 0.6 s–1, and 2.77 M–1, respectively. The long-term stability and excellent reproducibility of the proposed immunosensor indicates a suitable platform for detection of LDL or lipid molecules. This immunosensor provides an efficient platform for analysis of the antigen–antibody interactions of lipid molecules.
  • Editor: United States: American Chemical Society
  • Idioma: Inglês
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