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Sample Processing Impacts the Viability and Cultivability of the Sponge Microbiome

Esteves, Ana I S ; Amer, Nimra ; Nguyen, Mary ; Thomas, Torsten

Frontiers in microbiology, 2016-04, Vol.7, p.499-499 [Periódico revisado por pares]

Switzerland: Frontiers Media S.A

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  • Título:
    Sample Processing Impacts the Viability and Cultivability of the Sponge Microbiome
  • Autor: Esteves, Ana I S ; Amer, Nimra ; Nguyen, Mary ; Thomas, Torsten
  • Assuntos: Bacterial viability ; cultivation bias ; microbial cultivation ; Microbial Diversity ; Microbiology ; sponge microbiome
  • É parte de: Frontiers in microbiology, 2016-04, Vol.7, p.499-499
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
    Reviewed by: Klaus Jürgens, Leibniz Institute for Baltic Sea Research, Germany; Russell T. Hill, University of Maryland Center for Environmental Science, USA
    Edited by: Marcelino T. Suzuki, Sorbonne Universities (UPMC) and Centre National de la Recherche Scientifique, France
    This article was submitted to Aquatic Microbiology, a section of the journal Frontiers in Microbiology
  • Descrição: Sponges host complex microbial communities of recognized ecological and biotechnological importance. Extensive cultivation efforts have been made to isolate sponge bacteria, but most still elude cultivation. To identify the bottlenecks of sponge bacterial cultivation, we combined high-throughput 16S rRNA gene sequencing with a variety of cultivation media and incubation conditions. We aimed to determine the extent to which sample processing and cultivation conditions can impact bacterial viability and recovery in culture. We isolated 325 sponge bacteria from six specimens of Cymbastela concentrica and three specimens of Scopalina sp. These isolates were distributed over 37 different genera and 47 operational taxonomic units (defined at 97% 16S rRNA gene sequence identity). The cultivable bacterial community was highly specific to its sponge host and different media compositions yielded distinct microbial isolates. Around 97% of the isolates could be detected in the original sponge and represented a large but highly variable proportion (0.5-92% total abundance, depending on sponge species) of viable bacteria obtained after sample processing, as determined by propidium monoazide selective DNA modification of compromised cells. Our results show that the most abundant viable bacteria are also the most predominant groups found in cultivation, reflecting, to some extent, the relative abundances of the viable bacterial community, rather than the overall community estimated by direct molecular approaches. Cultivation is therefore shaped not only by the growth conditions provided, but also by the different cell viabilities of the bacteria that constitute the cultivation inoculum. These observations highlight the need to perform experiments to assess each method of sample processing for its accurate representation of the actual in situ bacterial community and its yield of viable cells.
  • Editor: Switzerland: Frontiers Media S.A
  • Idioma: Inglês
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