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Epigenetic alterations at genomic loci modified by gene targeting in Arabidopsis thaliana

Lieberman-Lazarovich, Michal ; Melamed-Bessudo, Cathy ; de Pater, Sylvia ; Levy, Avraham A Zhang, Xiaoyu

PloS one, 2013-12, Vol.8 (12), p.e85383-e85383 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Epigenetic alterations at genomic loci modified by gene targeting in Arabidopsis thaliana
Autor: Lieberman-Lazarovich, Michal ; Melamed-Bessudo, Cathy ; de Pater, Sylvia ; Levy, Avraham A
Zhang, Xiaoyu
Assuntos: Animals ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis thaliana ; Biological effects ; Biological research ; Biotechnology ; CRISPR ; Deoxyribonucleic acid ; DNA ; DNA methylation ; DNA Methylation - genetics ; DNA, Plant - genetics ; Engineering ; Epigenesis, Genetic ; Epigenetic inheritance ; Epigenetics ; Gene expression ; Gene loci ; Gene Targeting ; Gene therapy ; Genes ; Genetic Loci ; Genetics ; Genomes ; Genomics ; Homologous recombination ; Homology ; Methylation ; Mice ; Mutagenesis ; Mutation ; Nicotiana benthamiana ; Oryza ; Plant sciences ; Plants, Genetically Modified ; Seeds ; Stability
É parte de: PloS one, 2013-12, Vol.8 (12), p.e85383-e85383
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: The authors have declared that no competing interests exist.
Conceived and designed the experiments: MLL AAL. Performed the experiments: MLL CMB. Analyzed the data: MLL. Contributed reagents/materials/analysis tools: SP. Wrote the manuscript: MLL AAL.
Descrição: Gene Targeting (GT) is the integration of an introduced vector into a specific chromosomal site, via homologous recombination. It is considered an effective tool for precise genome editing, with far-reaching implications in biological research and biotechnology, and is widely used in mice, with the potential of becoming routine in many species. Nevertheless, the epigenetic status of the targeted allele remains largely unexplored. Using GT-modified lines of the model plant Arabidopsis thaliana, we show that the DNA methylation profile of the targeted locus is changed following GT. This effect is non-directional as methylation can be either completely lost, maintained with minor alterations or show instability in the generations subsequent to GT. As DNA methylation is known to be involved in several cellular processes, GT-related alterations may result in unexpected or even unnoticed perturbations. Our analysis shows that GT may be used as a new tool for generating epialleles, for example, to study the role of gene body methylation. In addition, the analysis of DNA methylation at the targeted locus may be utilized to investigate the mechanism of GT, many aspects of which are still unknown.
Editor: United States: Public Library of Science
Idioma: Inglês

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Epigenetic alterations at genomic loci modified by gene targeting in Arabidopsis thaliana

Lieberman-Lazarovich, Michal ; Melamed-Bessudo, Cathy ; de Pater, Sylvia ; Levy, Avraham A Zhang, Xiaoyu

United States: Public Library of Science

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