Idioma:

A method for studying protistan diversity using massively parallel sequencing of V9 hypervariable regions of small-subunit ribosomal RNA genes

Amaral-Zettler, Linda A ; McCliment, Elizabeth A ; Ducklow, Hugh W ; Huse, Susan M Langsley, Gordon

PloS one, 2009-07, Vol.4 (7), p.e6372-e6372 [Periódico revisado por pares]

United States: Public Library of Science

Texto completo disponível

Citações Citado por

Enviar para

Título:
A method for studying protistan diversity using massively parallel sequencing of V9 hypervariable regions of small-subunit ribosomal RNA genes
Autor: Amaral-Zettler, Linda A ; McCliment, Elizabeth A ; Ducklow, Hugh W ; Huse, Susan M
Langsley, Gordon
Assuntos: Analysis ; Bacteria ; Base Sequence ; Biodiversity ; Biological diversity ; Biometrics ; Deoxyribonucleic acid ; DNA ; DNA Primers ; Ecological research ; Ecology/Community Ecology and Biodiversity ; Ecosystem biology ; Ecosystems ; Estimates ; Eukaryotes ; Evolution ; Freshwater environments ; Gene sequencing ; Genes ; Internet resources ; Laboratories ; Marine and Aquatic Sciences/Genetics, Genomics, and Barcoding ; Methods ; Microbiology/Environmental Microbiology ; Microorganisms ; Molecular biology ; Multiprocessing ; Nucleotides ; Polymerase Chain Reaction ; Population ; Populations ; Power plants ; Ribonucleic acid ; Ribosomal RNA ; RNA ; RNA, Ribosomal - genetics ; rRNA ; Species richness ; Taxonomy
É parte de: PloS one, 2009-07, Vol.4 (7), p.e6372-e6372
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Conceived and designed the experiments: LAZ EAM. Performed the experiments: EAM. Analyzed the data: LAZ EAM SMH. Contributed reagents/materials/analysis tools: LAZ. Wrote the paper: LAZ EAM HWD SMH.
Descrição: Massively parallel pyrosequencing of amplicons from the V6 hypervariable regions of small-subunit (SSU) ribosomal RNA (rRNA) genes is commonly used to assess diversity and richness in bacterial and archaeal populations. Recent advances in pyrosequencing technology provide read lengths of up to 240 nucleotides. Amplicon pyrosequencing can now be applied to longer variable regions of the SSU rRNA gene including the V9 region in eukaryotes. We present a protocol for the amplicon pyrosequencing of V9 regions for eukaryotic environmental samples for biodiversity inventories and species richness estimation. The International Census of Marine Microbes (ICoMM) and the Microbial Inventory Research Across Diverse Aquatic Long Term Ecological Research Sites (MIRADA-LTERs) projects are already employing this protocol for tag sequencing of eukaryotic samples in a wide diversity of both marine and freshwater environments. Massively parallel pyrosequencing of eukaryotic V9 hypervariable regions of SSU rRNA genes provides a means of estimating species richness from deeply-sampled populations and for discovering novel species from the environment.
Editor: United States: Public Library of Science
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

A method for studying protistan diversity using massively parallel sequencing of V9 hypervariable regions of small-subunit ribosomal RNA genes

Amaral-Zettler, Linda A ; McCliment, Elizabeth A ; Ducklow, Hugh W ; Huse, Susan M Langsley, Gordon

United States: Public Library of Science

Buscando em bases de dados remotas. Favor aguardar.