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A liquid chromatography tandem mass spectrometry method for simultaneous determination of acid/alkaline phytohormones in grapes

Han, Zheng ; Liu, Gang ; Rao, Qinxiong ; Bai, Bing ; Zhao, Zhihui ; Liu, Hong ; Wu, Aibo

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2012-01, Vol.881 (15), p.83-89 [Periódico revisado por pares]

Amsterdam: Elsevier B.V

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Título:
A liquid chromatography tandem mass spectrometry method for simultaneous determination of acid/alkaline phytohormones in grapes
Autor: Han, Zheng ; Liu, Gang ; Rao, Qinxiong ; Bai, Bing ; Zhao, Zhihui ; Liu, Hong ; Wu, Aibo
Assuntos: acetonitrile ; Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Chromatography ; Chromatography, High Pressure Liquid - methods ; Drug Residues - analysis ; Drug Residues - isolation & purification ; Drug Stability ; formic acid ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Gibberellic acid ; Gibberellins - analysis ; Gibberellins - isolation & purification ; Grapes ; indole acetic acid ; indole butyric acid ; Indoleacetic Acids - analysis ; Indoleacetic Acids - isolation & purification ; Indoles - analysis ; Indoles - isolation & purification ; Ionization ; isopentenyladenine ; Isopentenyladenosine - analysis ; Isopentenyladenosine - isolation & purification ; Linearity ; Liquid chromatography ; Liquid chromatography tandem mass spectrometry ; Mass spectrometry ; Medical sciences ; methanol ; Methyl alcohol ; monitoring ; Multiresidue analysis ; naphthaleneacetic acid ; Naphthols - analysis ; Naphthols - isolation & purification ; Pharmacology. Drug treatments ; Phytohormone ; Plant Extracts - chemistry ; Plant Growth Regulators - analysis ; Plant Growth Regulators - isolation & purification ; rapid methods ; Reproducibility of Results ; Sensitivity and Specificity ; Solid Phase Extraction - instrumentation ; Solid Phase Extraction - methods ; spectrometers ; tandem mass spectrometry ; Tandem Mass Spectrometry - methods ; Vitis - chemistry
É parte de: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2012-01, Vol.881 (15), p.83-89
Notas: http://dx.doi.org/10.1016/j.jchromb.2011.12.002
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Descrição: ► A rapid LC–MS/MS for simultaneous determination of acid/alkaline phytohormones was developed. ► The procedures for sample preparation were thoroughly optimized. ► The performances of linearity, sensitivity, precision and matrix effects were satisfactory. ► The method was proved to be capable of rapid multiresidue analysis of multiclass phytohormones. A high performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) method for simultaneous determination of five acid/alkaline phytohormones, i.e., indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), naphthylacetic acid (NAA), gibberellic acid (GA 3) and isopentenyladenine (2IP), in grapes was developed. After optimization, the samples were extracted with methanol containing 1% formic acid and purified by Oasis HLB SPE cartridges. The analytes were separated on a Thermo Hypersil Gold column (100 mm × 2.1 mm, 3.0 μm) with water and acetonitrile, then determined with Thermo tandem quadrupole mass spectrometer operating in negative electro-spray ionization using selected reaction monitoring (SRM) mode. The established method was further validated by determining the linearity ( R 2 ≥ 0.9990), average recovery (82.5–105.4%), sensitivity (0.05–1.00 ng mL −1), precision (RSD ≤ 13.0%) and stability (RSD ≥ 82.0%). Finally, the application of the approach proposed to thirty grape samples convinced its desirable performance for rapid analysis of multiclass phytohormones, supporting its sufficient capability for multiresidue analyses or other analytical system targeting phytohormones in agriculture field.
Editor: Amsterdam: Elsevier B.V
Idioma: Inglês

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A liquid chromatography tandem mass spectrometry method for simultaneous determination of acid/alkaline phytohormones in grapes

Han, Zheng ; Liu, Gang ; Rao, Qinxiong ; Bai, Bing ; Zhao, Zhihui ; Liu, Hong ; Wu, Aibo

Amsterdam: Elsevier B.V

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