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A Small Non-Coding RNA Mediates Transcript Stability and Expression of Cytochrome bd Ubiquinol Oxidase Subunit I in Rickettsia conorii

Narra, Hema P ; Alsing, Jessica ; Sahni, Abha ; Montini, Michelle ; Zafar, Yasim ; Sahni, Sanjeev K

International journal of molecular sciences, 2023-02, Vol.24 (4), p.4008 [Periódico revisado por pares]

Switzerland: MDPI AG

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Título:
A Small Non-Coding RNA Mediates Transcript Stability and Expression of Cytochrome bd Ubiquinol Oxidase Subunit I in Rickettsia conorii
Autor: Narra, Hema P ; Alsing, Jessica ; Sahni, Abha ; Montini, Michelle ; Zafar, Yasim ; Sahni, Sanjeev K
Assuntos: Amino acids ; Animals ; Bacteria ; Binding ; Biosynthesis ; Cloning ; CydAB ; Cytochrome ; Cytochrome bd ; cytochrome bd oxidases ; Cytochromes - genetics ; E coli ; Fluorescence ; Gene expression ; Gene Expression Regulation, Bacterial ; Gene regulation ; Genes ; host–pathogen interaction ; Humans ; Infections ; Lungs ; Mammals - metabolism ; Mice ; non-coding RNA ; Oxidase ; Pathogens ; Plasmids ; Post-transcription ; Protein expression ; Proteins ; Rickettsia conorii ; Rickettsia conorii - genetics ; Rickettsia conorii - metabolism ; RNA, Messenger ; RNA, Small Untranslated - genetics ; small RNA mediated gene regulation ; Stability ; Ubiquinol ; Ubiquinol oxidase ; Virulence
É parte de: International journal of molecular sciences, 2023-02, Vol.24 (4), p.4008
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Small regulatory RNAs (sRNAs) are now widely recognized for their role in the post-transcriptional regulation of bacterial virulence and growth. We have previously demonstrated the biogenesis and differential expression of several sRNAs in during interactions with the human host and arthropod vector, as well as the in vitro binding of sRNA _sR42 to bicistronic cytochrome bd ubiquinol oxidase subunits I and II ( ) mRNA. However, the mechanism of regulation and the effect of sRNA binding on the stability of the bicistronic transcript and the expression of the and genes are still unknown. In this study, we determined the expression dynamics of _sR42 and its cognate target genes, and , in mouse lung and brain tissues during infection in vivo and employed fluorescent and reporter assays to decode the role of sRNA in regulating cognate gene transcripts. Quantitative RT-PCR revealed significant changes in the expression of sRNA and its cognate target gene transcripts during infection in vivo, and a greater abundance of these transcripts was observed in the lungs compared to brain tissue. Interestingly, while _sR42 and exhibited similar patterns of change in their expression, indicating the influence of sRNA on the mRNA target, the expression of was independent of sRNA expression. Further, we constructed reporter plasmids of sRNA and bicistronic mRNA to decipher the role of sRNA on CydA and CydB expression. We observed increased expression of CydA in the presence of sRNA but detected no change in CydB expression in the presence or absence of sRNA. In sum, our results demonstrate that the binding of _sR42 is required for the regulation of but not . Further studies on understanding the influence of this interaction on the mammalian host and tick vector during infection are in progress.
Editor: Switzerland: MDPI AG
Idioma: Inglês

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A Small Non-Coding RNA Mediates Transcript Stability and Expression of Cytochrome bd Ubiquinol Oxidase Subunit I in Rickettsia conorii

Narra, Hema P ; Alsing, Jessica ; Sahni, Abha ; Montini, Michelle ; Zafar, Yasim ; Sahni, Sanjeev K

Switzerland: MDPI AG

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