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ε, a New Subunit of RNA Polymerase Found in Gram-Positive Bacteria

Keller, Andrew N ; Yang, Xiao ; Wiedermannová, Jana ; Delumeau, Olivier ; Krásný, Libor ; Lewis, Peter J

Journal of bacteriology, 2014-10, Vol.196 (20), p.3622-3632 [Periódico revisado por pares]

United States: American Society for Microbiology

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Título:
ε, a New Subunit of RNA Polymerase Found in Gram-Positive Bacteria
Autor: Keller, Andrew N ; Yang, Xiao ; Wiedermannová, Jana ; Delumeau, Olivier ; Krásný, Libor ; Lewis, Peter J
Assuntos: Amino Acid Sequence ; Animals ; Bacillus subtilis - enzymology ; bacteriology ; bacteriophages ; DNA ; DNA-directed RNA polymerase ; DNA-Directed RNA Polymerases - chemistry ; DNA-Directed RNA Polymerases - genetics ; DNA-Directed RNA Polymerases - metabolism ; Firmicutes ; gene expression ; Gene Expression Regulation, Bacterial ; Gene Expression Regulation, Enzymologic ; Gram-positive bacteria ; Life Sciences ; Models, Molecular ; Molecular Sequence Data ; phenotype ; Phylogeny ; Protein Conformation ; Protein Subunits ; proteins ; transcription (genetics) ; X-ray diffraction
É parte de: Journal of bacteriology, 2014-10, Vol.196 (20), p.3622-3632
Notas: http://dx.doi.org/10.1128/JB.02020-14
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SourceType-Scholarly Journals-1
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PMCID: PMC4187704
Descrição: RNA polymerase in bacteria is a multisubunit protein complex that is essential for gene expression. We have identified a new subunit of RNA polymerase present in the high-A+T Firmicutes phylum of Gram-positive bacteria and have named it ε. Previously ε had been identified as a small protein (ω1) that copurified with RNA polymerase. We have solved the structure of ε by X-ray crystallography and show that it is not an ω subunit. Rather, ε bears remarkable similarity to the Gp2 family of phage proteins involved in the inhibition of host cell transcription following infection. Deletion of ε shows no phenotype and has no effect on the transcriptional profile of the cell. Determination of the location of ε within the assembly of RNA polymerase core by single-particle analysis suggests that it binds toward the downstream side of the DNA binding cleft. Due to the structural similarity of ε with Gp2 and the fact they bind similar regions of RNA polymerase, we hypothesize that ε may serve a role in protection from phage infection.
Editor: United States: American Society for Microbiology
Idioma: Inglês

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ε, a New Subunit of RNA Polymerase Found in Gram-Positive Bacteria

Keller, Andrew N ; Yang, Xiao ; Wiedermannová, Jana ; Delumeau, Olivier ; Krásný, Libor ; Lewis, Peter J

United States: American Society for Microbiology

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