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Acanthamoeba protease activity promotes allergic airway inflammation via protease-activated receptor 2

Park, Mi Kyung ; Cho, Min Kyoung ; Kang, Shin Ae ; Park, Hye-Kyung ; Kim, Dong-Hee ; Yu, Hak Sun Poojary, Venuprasad K.

PloS one, 2014-03, Vol.9 (3), p.e92726-e92726 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Acanthamoeba protease activity promotes allergic airway inflammation via protease-activated receptor 2
Autor: Park, Mi Kyung ; Cho, Min Kyoung ; Kang, Shin Ae ; Park, Hye-Kyung ; Kim, Dong-Hee ; Yu, Hak Sun
Poojary, Venuprasad K.
Assuntos: Acanthamoeba ; Acanthamoeba - enzymology ; Acanthamoeba - immunology ; Allergens ; Allergies ; Amebiasis - genetics ; Amebiasis - immunology ; Amebiasis - metabolism ; Amebiasis - parasitology ; Amoeba ; Animals ; Antibodies, Protozoan - immunology ; Antigens ; Antigens, Protozoan - immunology ; Asthma ; Asthma - immunology ; Asthma - metabolism ; Asthma - parasitology ; B cells ; Biology and Life Sciences ; CCL17 protein ; CCL22 protein ; Cell Line ; Chemokines ; Chemokines - biosynthesis ; Cytokines ; Dendritic cells ; Dendritic Cells - immunology ; Dendritic Cells - metabolism ; Disease Models, Animal ; Drug dosages ; Dust control ; Epithelial cells ; Female ; Fluoride ; Fluorides ; Gene expression ; House dust ; Humans ; Immune response ; Immunoglobulin E ; Immunoglobulin E - immunology ; Inflammation ; Inoculation ; Interleukin 4 ; Laboratory animals ; Lung - immunology ; Lung - metabolism ; Lung - parasitology ; Lungs ; Lymphocytes ; Lymphocytes T ; Medicine ; Medicine and Health Sciences ; Mice ; Parasitology ; Peptide Hydrolases - metabolism ; Protease ; Protease inhibitors ; Proteases ; Proteins ; Receptor, PAR-2 - genetics ; Receptor, PAR-2 - metabolism ; Research and Analysis Methods ; Respiratory Hypersensitivity - genetics ; Respiratory Hypersensitivity - immunology ; Respiratory Hypersensitivity - metabolism ; Respiratory Hypersensitivity - parasitology ; Respiratory tract ; Respiratory tract diseases ; Serine ; Serine proteinase ; T cells ; Th2 Cells - immunology ; Th2 Cells - metabolism ; Thrombin ; Thymic stromal lymphopoietin ; Trophozoites
É parte de: PloS one, 2014-03, Vol.9 (3), p.e92726-e92726
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
Conceived and designed the experiments: MKP HKP DHK HSY. Performed the experiments: MKP HKP MKC SAK. Analyzed the data: MKP HKP MKC SAK DHK. Contributed reagents/materials/analysis tools: MKP HKP MKC SAK DHK HSY. Wrote the paper: MKP HKP MKC DHK HSY.
Competing Interests: The authors have declared that no competing interests exist.
Descrição: Acanthamoeba is a free-living amoeba commonly present in the environment and often found in human airway cavities. Acanthamoeba possesses strong proteases that can elicit allergic airway inflammation. To our knowledge, the aeroallergenicity of Acanthamoeba has not been reported. We repeatedly inoculated mice with Acanthamoeba trophozoites or excretory-secretory (ES) proteins intra-nasally and evaluated symptoms and airway immune responses. Acanthamoeba trophozoites or ES proteins elicited immune responses in mice that resembled allergic airway inflammation. ES proteins had strong protease activity and activated the expression of several chemokine genes (CCL11, CCL17, CCL22, TSLP, and IL-25) in mouse lung epithelial cells. The serine protease inhibitor phenyl-methane-sulfonyl fluoride (PMSF) inhibited ES protein activity. ES proteins also stimulated dendritic cells and enhanced the differentiation of naive T cells into IL-4-secreting T cells. After repeated inoculation of the protease-activated receptor 2 knockout mouse with ES proteins, airway inflammation and Th2 immune responses were markedly reduced, but not to basal levels. Furthermore, asthma patients had higher Acanthamoeba-specific IgE titers than healthy controls and we found Acanthamoeba specific antigen from house dust in typical living room. Our findings suggest that Acanthamoeba elicits allergic airway symptoms in mice via a protease allergen. In addition, it is possible that Acanthamoeba may be one of the triggers human airway allergic disease.
Editor: United States: Public Library of Science
Idioma: Inglês

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Acanthamoeba protease activity promotes allergic airway inflammation via protease-activated receptor 2

Park, Mi Kyung ; Cho, Min Kyoung ; Kang, Shin Ae ; Park, Hye-Kyung ; Kim, Dong-Hee ; Yu, Hak Sun Poojary, Venuprasad K.

United States: Public Library of Science

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