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Evidence for an Antiapoptotic Role of Dopamine in Developing Retinal Tissue

Varella, Marcia H. ; De Mello, Fernando G. ; Linden, Rafael

Journal of neurochemistry, 1999-08, Vol.73 (2), p.485-492 [Periódico revisado por pares]

Oxford UK: Blackwell Science Ltd

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Título:
Evidence for an Antiapoptotic Role of Dopamine in Developing Retinal Tissue
Autor: Varella, Marcia H. ; De Mello, Fernando G. ; Linden, Rafael
Assuntos: Animals ; Animals, Newborn ; Anisomycin - pharmacology ; Apoptosis - drug effects ; Apoptosis - physiology ; Benzazepines - pharmacology ; Biological and medical sciences ; Central nervous system ; Colforsin - pharmacology ; Cyclic AMP ; Cyclic AMP - metabolism ; Cyclic AMP-Dependent Protein Kinases - metabolism ; Cyclic AMP‐dependent protein kinase ; Dopamine - pharmacology ; Dopamine - physiology ; Dopamine Agonists - pharmacology ; Dopamine Antagonists - pharmacology ; Eye and associated structures. Visual pathways and centers. Vision ; Fundamental and applied biological sciences. Psychology ; Neurogenesis ; Programmed cell death ; Protein Synthesis Inhibitors - pharmacology ; Rats ; Rats, Inbred Strains ; Retina - cytology ; Retina - enzymology ; Retina - growth & development ; Signal Transduction - drug effects ; Signal Transduction - physiology ; Spiperone - pharmacology ; Vertebrates: nervous system and sense organs
É parte de: Journal of neurochemistry, 1999-08, Vol.73 (2), p.485-492
Notas: p
ADTN, (±)‐2‐amino‐6,7‐dihydroxy‐1,2,3,4‐tetrahydronaphthalene ; cAMP, cyclic AMP ; 6‐Cl‐PB, 6‐chloro‐7,8‐dihydroxy‐1‐phenyl‐2,3,4,5‐tetrahydro‐1
2
H
3‐benzazepine ; H‐89
bromocinnamylamino)ethyl]‐5‐isoquinolinesulfonamide ; NBL, neuroblastic layer ; P, postnatal day ; PKA, cyclic AMP‐dependent protein kinase.
N
Abbreviations used
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Descrição: : Inhibition of protein synthesis leads to apoptosis in the undifferentiated neuroblastic layer of the retina of newborn rats. We have shown previously that an increase in the intracellular concentration of cyclic AMP prevented apoptosis induced in the retinal neuroblastic layer by inhibition of protein synthesis. In this study, we tested the effects of dopamine on retinal apoptosis and related these effects to the intracellular concentration of cyclic AMP. Both dopamine (100 μM) and the D1‐like agonists SKF‐38393, 6‐chloro‐7,8‐dihydroxy‐1‐phenyl‐2,3,4,5‐tetrahydro‐1H‐3‐benzazepine (6‐Cl‐PB), and (±)‐2‐amino‐6,7‐dihydroxy‐1,2,3,4‐tetrahydronapthalene (100 μM) blocked apoptosis induced in the neuroblastic layer by the protein synthesis inhibitor anisomycin. The antiapoptotic effects of the D1‐like agonists were not reversed by the D1‐like antagonist SCH‐23390 (5‐100 μM). Both dopamine and D1‐like angonists induced a five‐to sevenfold increase in the intracellular concentration of cyclic AMP in the retina of newborn rats. The concentration of cyclic AMP induced by the D1‐like agonists in the presence of 100 μM SCH‐23390 was still at least two‐ to threefold as high as control values, showing that the activation of adenylyl cyclase by D1‐like agonists was reversed only partially by the specific antagonist. The isoquinolinesulfonamide H‐89 (20 μM), an inhibitor of cyclic AMP‐dependent protein kinase, partially prevented the antiapoptotic effect of 6‐Cl‐PB. The data show that an early effect of dopamine in the developing retina is the control of programmed cell death. The antiapoptotic effect of dopamine is mediated, at least in part, through an atypical D1‐like receptor coupled to stimulation of adenylyl cyclase, followed by activation of cyclic AMP‐dependent protein kinase.
Editor: Oxford UK: Blackwell Science Ltd
Idioma: Inglês

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Evidence for an Antiapoptotic Role of Dopamine in Developing Retinal Tissue

Varella, Marcia H. ; De Mello, Fernando G. ; Linden, Rafael

Oxford UK: Blackwell Science Ltd

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