skip to main content
Idioma:
Tipo de recurso Mostra resultados com: Mostra resultados com: Índice

Pharmacological Mechanism of Aucklandiae Radix against Gastric Ulcer Based on Network Pharmacology and In Vivo Experiment

Feng, Lan ; A, Lisha ; Li, Huifang ; Mu, Xiyele ; Ta, Na ; Bai, Laxinamujila ; Fu, Minghai ; Chen, Yongsheng

Medicina (Kaunas, Lithuania), 2023-03, Vol.59 (4), p.666 [Periódico revisado por pares]

Switzerland: MDPI AG

Texto completo disponível

Citações Citado por
  • Título:
    Pharmacological Mechanism of Aucklandiae Radix against Gastric Ulcer Based on Network Pharmacology and In Vivo Experiment
  • Autor: Feng, Lan ; A, Lisha ; Li, Huifang ; Mu, Xiyele ; Ta, Na ; Bai, Laxinamujila ; Fu, Minghai ; Chen, Yongsheng
  • Assuntos: Animals ; Aucklandiae Radix ; Biotechnology industry ; Chinese medicine ; Cyclooxygenase 2 ; Digestive system ; Drug dosages ; Ethanol ; gastric ulcer ; Herbal medicine ; Medical research ; molecular docking ; Molecular Docking Simulation ; network analysis ; Network Pharmacology ; pharmacological mechanism ; Pharmacology ; Proteins ; Rats ; Stomach Ulcer - drug therapy ; Tumor Necrosis Factor-alpha ; Tumor necrosis factor-TNF ; Ulcers
  • É parte de: Medicina (Kaunas, Lithuania), 2023-03, Vol.59 (4), p.666
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
    These authors contributed equally to this work.
  • Descrição: : is a well-known medicinal herb that is often used to treat gastric ulcer, but its molecular mechanism of anti-ulcer action is poorly understood. This research aimed to reveal the potential active components, core targets, and mechanisms of in treating gastric ulcer by combining network pharmacology and animal experimentation. : First, a network pharmacology strategy was used to predict the main components, candidate targets, and potential signaling pathways. Molecular docking was then used to confirm the binding affinity between the main components and primary targets. Finally, rats were treated with indomethacin 30 mg/kg to establish a gastric ulcer model. extract (0.15, 0.3, and 0.6 g/kg) was pre-treated in rats by oral gavage for 14 days, and the protective effect and candidate targets of network pharmacology were validated through morphological observation, pathological staining, and biochemical index detection. : A total of eight potential active components and 331 predicted targets were screened from , 37 of which were common targets with gastric ulcer. According to the component-target network and protein-protein interaction (PPI) network, stigmasterol, mairin, sitosterol, and dehydrocostus lactone were identified as the key components, and RAC-alpha serine/threonine-protein kinase (AKT1), prostaglandin-endoperoxide synthase 2 (PTGS2), interleukin 1 beta (IL1B), caspase-3 (CASP3), and CASP8 were selected as the core targets. Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment results revealed the pharmacological mechanism of against gastric ulcer related to many biological processes and pathways, including antibacterial, anti-inflammatory, prostaglandin receptor response, and apoptosis. Molecular docking verification showed that the key components and core targets had good binding affinities. In the in vivo experiments, notably relieved the gastric ulcer by reducing the levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and myeloperoxidase (MPO) while improving the gastric histopathological features. : The overall findings suggest that treats gastric ulcer with a multi-component, multi-target, and multi-mechanism model.
  • Editor: Switzerland: MDPI AG
  • Idioma: Inglês
Links
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.