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The effects of permeating cryoprotectants on intracellular free-calcium concentrations and developmental potential of in vitro-matured feline oocytes

Herrick, Jason R ; Wang, Chunmin ; Machaty, Zoltan

Reproduction fertility and development, 2016-01, Vol.28 (5), p.599-607 [Periódico revisado por pares]

Australia

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Título:
The effects of permeating cryoprotectants on intracellular free-calcium concentrations and developmental potential of in vitro-matured feline oocytes
Autor: Herrick, Jason R ; Wang, Chunmin ; Machaty, Zoltan
Assuntos: Animals ; Blastocyst - drug effects ; Blastocyst - metabolism ; Blastocyst - pathology ; Calcium - metabolism ; Cats ; Cell Membrane Permeability ; Cells, Cultured ; Cleavage Stage, Ovum - drug effects ; Cryopreservation ; Cryoprotective Agents - pharmacology ; Cryoprotective Agents - toxicity ; Dimethyl Sulfoxide - pharmacology ; Embryo Culture Techniques ; Ethylene Glycol - pharmacology ; Female ; Fertilization in Vitro ; In Vitro Oocyte Maturation Techniques ; Oocytes - drug effects ; Oocytes - metabolism ; Oocytes - pathology ; Parthenogenesis - drug effects ; Propylene Glycols - pharmacology ; Vitrification
É parte de: Reproduction fertility and development, 2016-01, Vol.28 (5), p.599-607
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Embryos produced from vitrified feline oocytes have resulted in pregnancies, but the efficiency of oocyte vitrification in cats is still low. Our objectives were to evaluate the effects of exposing feline oocytes to ethylene glycol (EG), propanediol (PrOH) and dimethyl sulfoxide (DMSO) on changes in intracellular free-calcium concentrations ([Ca(2+)]i), the time needed for enzymatic digestion of the zona pellucida (ZP), the incidence of parthenogenetic activation and degeneration and embryonic development following in vitro fertilisation (IVF). All of the chemicals tested altered [Ca(2+)]i, but changes in [Ca(2+)]i, resistance of the ZP to enzymatic digestion and the incidence of parthenogenetic activation (<5% for all treatments) were not affected (P>0.05) by extracellular Ca(2+). Exposure to EG (>44.1%) and DMSO (19.7%) increased (P<0.05) oocyte degeneration compared with control oocytes and oocytes exposed to PrOH (≤2.5%). Following exposure to a combination of PrOH and DMSO (10% v/v each), blastocyst development (per cleaved embryo; 52.1%) was similar (P>0.05) to control oocytes (64.4%). When oocytes were vitrified with PrOH and DMSO, 28.3% of surviving (intact plasma membrane) oocytes cleaved following IVF, but no blastocyst developed. When a non-permeating cryoprotectant (galactose, 0.25M) was added to the vitrification medium, 47.7% of surviving oocytes cleaved and 14.3% developed to the blastocyst stage.
Editor: Australia
Idioma: Inglês

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The effects of permeating cryoprotectants on intracellular free-calcium concentrations and developmental potential of in vitro-matured feline oocytes

Herrick, Jason R ; Wang, Chunmin ; Machaty, Zoltan

Australia

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