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Development of three-dimensional collagen scaffolds with controlled architecture for cell migration studies using breast cancer cell lines

Campbell, Jonathan J ; Husmann, Anke ; Hume, Robert D ; Watson, Christine J ; Cameron, Ruth E

Biomaterials, 2017-01, Vol.114, p.34-43 [Periódico revisado por pares]

Netherlands: Elsevier Ltd

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Título:
Development of three-dimensional collagen scaffolds with controlled architecture for cell migration studies using breast cancer cell lines
Autor: Campbell, Jonathan J ; Husmann, Anke ; Hume, Robert D ; Watson, Christine J ; Cameron, Ruth E
Assuntos: Advanced Basic Science ; Anisotropy ; Architecture ; Assaying ; Biomimetic Materials - chemical synthesis ; Biotechnology ; Breast cancer ; Breast Neoplasms - pathology ; Breast Neoplasms - physiopathology ; Cancer ; Cell Adhesion ; Cell Line, Tumor ; Cell Movement ; Collagen - chemistry ; Collagen 1 ; Dentistry ; Equipment Design ; Extracellular Matrix - chemistry ; Humans ; Ice-templating technique ; In vitro testing ; Invasion ; MCF-7 Cells ; Migration ; Printing, Three-Dimensional ; Scaffold architecture ; Scaffolds ; Three dimensional migration ; Tissue Array Analysis - instrumentation ; Tissue Engineering - instrumentation ; Tissue Scaffolds
É parte de: Biomaterials, 2017-01, Vol.114, p.34-43
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: Abstract Cancer is characterised by cell heterogeneity and the development of 3D in vitro assays that can distinguish more invasive or migratory phenotypes could enhance diagnosis or drug discovery. 3D collagen scaffolds have been used to develop analogues of complex tissues in vitro and are suited to routine biochemical and immunological assays. We sought to increase 3D model tractability and modulate the migration rate of seeded cells using an ice-templating technique to create either directional/anisotropic or isotropic porous architectures within cross-linked collagen scaffolds. Anisotropic scaffolds supported the enhanced migration of an invasive breast cancer cell line MDA-MB-231 with an altered spatial distribution of proliferative cells in contrast to invasive MDA-MB-468 and the non-invasive MCF-7 cells lines. In addition, MDA-MB-468 showed increased migration upon epithelial-to-mesenchymal transition (EMT) in anisotropic scaffolds. The provision of controlled architecture in this system may act both to increase assay robustness and as a tuneable parameter to capture detection of a migrated population within a set time, with consequences for primary tumour migration analysis. The separation of invasive clones from a cancer biomass with in vitro platforms could enhance drug development and diagnosis testing by contributing assay metrics including migration rate, as well as modelling cell-cell and cell-matrix interaction in a system compatible with routine histopathological testing.
Editor: Netherlands: Elsevier Ltd
Idioma: Inglês

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Development of three-dimensional collagen scaffolds with controlled architecture for cell migration studies using breast cancer cell lines

Campbell, Jonathan J ; Husmann, Anke ; Hume, Robert D ; Watson, Christine J ; Cameron, Ruth E

Netherlands: Elsevier Ltd

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