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2′,4′-BNA/LNA aptamers: CE-SELEX using a DNA-based library of full-length 2′-O,4′-C-methylene-bridged/linked bicyclic ribonucleotides

Kasahara, Yuuya ; Irisawa, Yuuta ; Ozaki, Hiroaki ; Obika, Satoshi ; Kuwahara, Masayasu

Bioorganic & medicinal chemistry letters, 2013-03, Vol.23 (5), p.1288-1292 [Periódico revisado por pares]

England: Elsevier Ltd

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Título:
2′,4′-BNA/LNA aptamers: CE-SELEX using a DNA-based library of full-length 2′-O,4′-C-methylene-bridged/linked bicyclic ribonucleotides
Autor: Kasahara, Yuuya ; Irisawa, Yuuta ; Ozaki, Hiroaki ; Obika, Satoshi ; Kuwahara, Masayasu
Assuntos: Aptamers, Nucleotide - chemistry ; Aptamers, Nucleotide - genetics ; Aptamers, Nucleotide - metabolism ; Bridged (locked) nucleic acid aptamer ; Bridged-Ring Compounds - chemistry ; Capillary electrophoresis-systematic evolution of ligands by exponential enrichment (CE-SELEX) ; DNA-Directed DNA Polymerase - chemistry ; DNA-Directed DNA Polymerase - genetics ; Gene Library ; Humans ; KOD DNA polymerase ; Nucleotides - chemistry ; Oligonucleotides - chemistry ; Ribonucleotides - chemistry ; Ribonucleotides - genetics ; SELEX Aptamer Technique - methods ; Xeno-nucleic acid (XNA)
É parte de: Bioorganic & medicinal chemistry letters, 2013-03, Vol.23 (5), p.1288-1292
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: DNA-based aptamers that contain 2′-O,4′-C-methylene-bridged/linked bicyclic ribonucleotides (B/L nucleotides) over the entire length were successfully obtained using a capillary electrophoresis systematic evolution of ligands by exponential enrichment (CE-SELEX) method. A modified DNA library was prepared with an enzyme mix of KOD Dash and KOD mutant DNA polymerases. Forty 2′-O,4′-C-methylene bridged/locked nucleic acid (2′,4′-BNA/LNA) aptamers were isolated from an enriched pool and classified into six groups according to their sequence. 2′,4′-BNA/LNA aptamers of groups V and VI bound human thrombin with Kd values in the range of several 10 nanomolar levels.
Editor: England: Elsevier Ltd
Idioma: Inglês

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2′,4′-BNA/LNA aptamers: CE-SELEX using a DNA-based library of full-length 2′-O,4′-C-methylene-bridged/linked bicyclic ribonucleotides

Kasahara, Yuuya ; Irisawa, Yuuta ; Ozaki, Hiroaki ; Obika, Satoshi ; Kuwahara, Masayasu

England: Elsevier Ltd

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