skip to main content
Idioma:

Poly (ADP-ribose) polymerase methods and protocols

Alexei Tulin

New York Humana Springer c2011

Acesso online. A biblioteca também possui exemplares impressos.

  • Título:
    Poly (ADP-ribose) polymerase methods and protocols
  • Autor: Alexei Tulin
  • Assuntos: Poly(ADP-ribose) Polymerases; ENZIMAS; CICLO CELULAR; DNA POLIMERASES
  • Descrição: Approaches to detect PARP-1 activation in vivo, in situ, and in vitro Quantification of poly(ADP-ribose) in vitro : determination of the ADP-Ribose chain length and branching pattern Assay for protein modification by poly-ADP-Ribose in vitro Identification of distinct amino acids as ADP-Ribose acceptor sites by mass spectrometry Noncovalent protein interaction with poly(ADP-Ribose) Noncovalent pADPr interaction with proteins and competition with RNA for binding to proteins Affinity-based assays for the identification and quantitative evaluation of noncovalent poly(ADP-Ribose)-binding proteins Detecting and quantifying pADPr in vivo Purification of recombinant poly(ADP-Ribose) polymerases Methods for purification of proteins associated with cellular poly(ADP-Ribose) and PARP-specific poly(ADP-Ribose) Purification and characterization of poly(ADP-Ribosyl)ated DNA replication/repair complexes PARP1 genomics : chromatin immunoprecipitation approach using anti-PARP1 antibody (ChIP and ChIP-seq) Purification of human PARP-1 and PARP-1 domains from Escherichia coli for structural and biochemical analysis Regulation of chromatin structure by PARP-1 Use of PARP inhibitors in cancer therapy : use as adjuvant with chemotherapy or radiotherapy ; use as a single agent in susceptible patients ; techniques used to identify susceptible patients
    Quantification of PARP activity in human tissues : ex vivo assays in blood cells and immunohistochemistry in human biopsies Assays for hypermethylation of the BRCA1 gene promoter in tumor cells to predict sensitivity to PARP-inhibitor therapy Generation of poly(ADP-ribosyl)ation deficient mutants of the transcription factor, CTCF Phenotypic characterization of Parp-1 and Parp-2 deficient mice and cells Poly(ADP-ribosyl)ation pathways in mammals : the advantage of murine PARG null mutation Genetic approaches to targeting multiple PARP genes in a mammalian genome Approaches to separate and identify polyADP-Ribosylated proteins using poly(ADP-Ribose) glycohydrolase-knockout Drosophila Immunolocalization of poly ADP-Ribose on Drosophila polytene chromosomes Immunodetection of pADPr in Drosophila tissues : immunostaining of thick sections; immuno-EM Poly(ADP-Ribose) metabolism analysis in the Nematode Caenorhabditis elegans Genetic dissection of PARylation in the filamentous fungus Neurospora crassa Purification of the poly-ADP-Ribose polymerase-like thermozyme from the Archaeon Sulfolobus solfataricus Strategies employed for the development of PARP inhibitors Small-molecule collection and high-throughput colorimetric assay to identify PARP1 inhibitors
  • Títulos relacionados: Série:Methods in molecular biology (Clifton, N.J.) v. 780
  • Editor: New York Humana Springer
  • Data de criação/publicação: c2011
  • Formato: xv, 521 p ill.
  • Idioma: Inglês
Links
Voltar para lista de resultados
Ir para página anteriorAnterior Resultado  10 AvançarIr para próxima página

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.