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Direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry improves appropriateness of antibiotic treatment of bacteremia

Vlek, Anne L M ; Bonten, Marc J M ; Boel, C H Edwin Doherty, T. Mark

PloS one, 2012-03, Vol.7 (3), p.e32589-e32589 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry improves appropriateness of antibiotic treatment of bacteremia
Autor: Vlek, Anne L M ; Bonten, Marc J M ; Boel, C H Edwin
Doherty, T. Mark
Assuntos: Anti-Bacterial Agents - therapeutic use ; Antibiotics ; Antimicrobial agents ; Bacteremia ; Bacteremia - blood ; Bacteremia - drug therapy ; Bacteremia - microbiology ; Bacteria ; Bacterial Typing Techniques - methods ; Bacteriological Techniques ; Biology ; Blood ; Blood culture ; Blood tests ; Broths ; Candida ; Care and treatment ; Culture ; Desorption ; Gram-Negative Bacteria - isolation & purification ; Gram-Positive Bacteria - isolation & purification ; Gram-Positive Cocci - isolation & purification ; Hospitals ; Humans ; Identification ; Infections ; Information systems ; Intensive care units ; Intervention ; Ionization ; Ions ; Laboratories ; Lasers ; Mass spectrometry ; Mass spectroscopy ; Matrix assisted ; Medical examination ; Medicine ; Microbiology ; Microorganisms ; Opening hours ; Pathogens ; Patients ; Prospective Studies ; Scientific imaging ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; Spectroscopy
É parte de: PloS one, 2012-03, Vol.7 (3), p.e32589-e32589
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
Conceived and designed the experiments: AV EB MB. Performed the experiments: AV EB. Analyzed the data: AV EB MB. Contributed reagents/materials/analysis tools: AV EB MB. Wrote the paper: AV EB MB.
Descrição: Matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) allows the identification of microorganisms directly from positive blood culture broths. Use of the MALDI-TOF MS for rapid identification of microorganisms from blood culture broths can reduce the turnaround time to identification and may lead to earlier appropriate treatment of bacteremia. During February and April 2010, direct MALDI-TOF MS was routinely performed on all positive blood cultures. During December 2009 and March 2010 no direct MALDI-TOF MS was used. Information on antibiotic therapy was collected from the hospital and intensive care units' information systems from all positive blood cultures during the study period. In total, 253 episodes of bacteremia were included of which 89 during the intervention period and 164 during the control period. Direct performance of MALDI-TOF MS on positive blood culture broths reduced the time till species identification by 28.8-h and was associated with an 11.3% increase in the proportion of patients receiving appropriate antibiotic treatment 24 hours after blood culture positivity (64.0% in the control period versus 75.3% in the intervention period (p0.01)). Routine implementation of this technique increased the proportion of patients on adequate antimicrobial treatment within 24 hours.
Editor: United States: Public Library of Science
Idioma: Inglês

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Direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry improves appropriateness of antibiotic treatment of bacteremia

Vlek, Anne L M ; Bonten, Marc J M ; Boel, C H Edwin Doherty, T. Mark

United States: Public Library of Science

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