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Evaluation of reference genes of Mytilus galloprovincialis and Ruditapes philippinarum infected with three bacteria strains for gene expression analysis

Moreira, Rebeca ; Pereiro, Patricia ; Costa, María M. ; Figueras, Antonio ; Novoa, Beatriz

Aquatic living resources (Montrouge), 2014-07, Vol.27 (3-4), p.147-152 [Periódico revisado por pares]

Paris: EDP Sciences

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Título:
Evaluation of reference genes of Mytilus galloprovincialis and Ruditapes philippinarum infected with three bacteria strains for gene expression analysis
Autor: Moreira, Rebeca ; Pereiro, Patricia ; Costa, María M. ; Figueras, Antonio ; Novoa, Beatriz
Assuntos: Animal, plant and microbial ecology ; Applied ecology ; Biological and medical sciences ; Exploitation and management of natural biological resources (hunting, fishing and exploited populations survey, etc.) ; Fundamental and applied biological sciences. Psychology ; Marine ; Micrococcus lysodeikticus ; Mytilus galloprovincialis ; qPCR ; Reference (housekeeping) genes ; Ruditapes philippinarum ; Shellfish immunity ; Vibrio alginolyticus ; Vibrio anguillarum ; Vibrio spp
É parte de: Aquatic living resources (Montrouge), 2014-07, Vol.27 (3-4), p.147-152
Notas: istex:BD687193E6A141F418190B61634CFFA6846FDD3A
ark:/67375/80W-ZFCXHHDZ-R
publisher-ID:alr140015
PII:S0990744014000151
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Quantitative real-time polymerase chain reaction (qPCR) is probably the most used method for gene expression quantification because of its high sensitivity and specificity. Nevertheless, this technology can undergo experimental errors and variations. Normalization of the results using a reference gene is therefore necessary to minimize these variations. As the study of immune genes in bivalve mollusks has increased in the last years, the establishment of adequate and stable reference genes for bivalves is strongly required. We analyzed the behavior of four putative reference genes: ribosomal RNA 18S, actin, elongation factor 1 − α and α-tubulin. The suitability of these four genes as internal control for qPCR was evaluated in mussel (Mytilus galloprovincialis) and clam (Ruditapes philippinarum) hemocytes after bacterial challenge. Four independent approaches (BestKeeper, GeNorm, NormFinder and DeltaCt ) were used to assess the suitable genes for stable expression. For these particular circumstances, the most stable gene in hemocytes was elongation factor 1 − α for mussels and α-tubulin for clams.
Editor: Paris: EDP Sciences
Idioma: Inglês;Francês;Espanhol

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Evaluation of reference genes of Mytilus galloprovincialis and Ruditapes philippinarum infected with three bacteria strains for gene expression analysis

Moreira, Rebeca ; Pereiro, Patricia ; Costa, María M. ; Figueras, Antonio ; Novoa, Beatriz

Paris: EDP Sciences

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