Idioma:

Induction of immune mediators in glioma and prostate cancer cells by non-lethal photodynamic therapy

Kammerer, Robert ; Buchner, Alexander ; Palluch, Patrick ; Pongratz, Thomas ; Oboukhovskij, Konstantin ; Beyer, Wolfgang ; Johansson, Ann ; Stepp, Herbert ; Baumgartner, Reinhold ; Zimmermann, Wolfgang Najbauer, Joseph

PloS one, 2011-06, Vol.6 (6), p.e21834-e21834 [Periódico revisado por pares]

United States: Public Library of Science

Citações Citado por

Enviar para

Título:
Induction of immune mediators in glioma and prostate cancer cells by non-lethal photodynamic therapy
Autor: Kammerer, Robert ; Buchner, Alexander ; Palluch, Patrick ; Pongratz, Thomas ; Oboukhovskij, Konstantin ; Beyer, Wolfgang ; Johansson, Ann ; Stepp, Herbert ; Baumgartner, Reinhold ; Zimmermann, Wolfgang
Najbauer, Joseph
Assuntos: Androgens ; Animals ; Autocrine signalling ; Biology ; Brain cancer ; Brain tumors ; Cancer ; Cancer cells ; Cancer therapies ; Cell cycle ; Cell Line, Tumor ; Cellular stress response ; Chemokines - metabolism ; Coding ; Cytokines ; Deoxyribonucleic acid ; DNA ; DNA microarrays ; Drugs ; Gene expression ; Genes ; Genomes ; Glioblastoma ; Glioma - immunology ; Glioma cells ; Gliomas ; Humans ; Immune response ; Immunity ; Immunology ; Immunosuppressive agents ; Inflammation ; Interleukin 6 ; Interleukin 6 receptors ; Interleukin 8 ; Interleukin-6 - metabolism ; Kinases ; Laboratories ; Lasers ; Male ; Medicine ; Mice ; Mitochondria ; Neutrophils ; Oligonucleotides ; Ovarian cancer ; Pathways ; Phosphorylation ; Photochemotherapy ; Photodynamic therapy ; Photosensitization ; Prostate ; Prostate cancer ; Prostatic Neoplasms - immunology ; Proteins ; Receptors, Interleukin-6 - metabolism ; Tumor cells ; Tumors ; Wound healing
É parte de: PloS one, 2011-06, Vol.6 (6), p.e21834-e21834
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Conceived and designed the experiments: RK HS RB WZ. Performed the experiments: PP TP KO AJ WZ. Analyzed the data: RK AB PP TP KO WB AJ WZ. Wrote the paper: RK HS WZ. Statistical analysis: RK AB.
Descrição: Photodynamic therapy (PDT) uses the combination of photosensitizing drugs and harmless light to cause selective damage to tumor cells. PDT is therefore an option for focal therapy of localized disease or for otherwise unresectable tumors. In addition, there is increasing evidence that PDT can induce systemic anti-tumor immunity, supporting control of tumor cells, which were not eliminated by the primary treatment. However, the effect of non-lethal PDT on the behavior and malignant potential of tumor cells surviving PDT is molecularly not well defined. Here we have evaluated changes in the transcriptome of human glioblastoma (U87, U373) and human (PC-3, DU145) and murine prostate cancer cells (TRAMP-C1, TRAMP-C2) after non-lethal PDT in vitro and in vivo using oligonucleotide microarray analyses. We found that the overall response was similar between the different cell lines and photosensitizers both in vitro and in vivo. The most prominently upregulated genes encoded proteins that belong to pathways activated by cellular stress or are involved in cell cycle arrest. This response was similar to the rescue response of tumor cells following high-dose PDT. In contrast, tumor cells dealing with non-lethal PDT were found to significantly upregulate a number of immune genes, which included the chemokine genes CXCL2, CXCL3 and IL8/CXCL8 as well as the genes for IL6 and its receptor IL6R, which can stimulate proinflammatory reactions, while IL6 and IL6R can also enhance tumor growth. Our results indicate that PDT can support anti-tumor immune responses and is, therefore, a rational therapy even if tumor cells cannot be completely eliminated by primary phototoxic mechanisms alone. However, non-lethal PDT can also stimulate tumor growth-promoting autocrine loops, as seen by the upregulation of IL6 and its receptor. Thus the efficacy of PDT to treat tumors may be improved by controlling unwanted and potentially deleterious growth-stimulatory pathways.
Editor: United States: Public Library of Science
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Realização: Logos de Redes Sociais:

Induction of immune mediators in glioma and prostate cancer cells by non-lethal photodynamic therapy

Kammerer, Robert ; Buchner, Alexander ; Palluch, Patrick ; Pongratz, Thomas ; Oboukhovskij, Konstantin ; Beyer, Wolfgang ; Johansson, Ann ; Stepp, Herbert ; Baumgartner, Reinhold ; Zimmermann, Wolfgang Najbauer, Joseph

United States: Public Library of Science

Buscando em bases de dados remotas. Favor aguardar.