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Photodynamic inactivation for in vitro decontamination of Staphylococcus aureus in whole blood

Corrêa, Thaila Quatrini ; Blanco, Kate Cristina ; Soares, Jennifer Machado ; Inada, Natalia Mayumi ; Kurachi, Cristina ; Golim, Marjorie de Assis ; Deffune, Elenice ; Bagnato, Vanderlei Salvador

Photodiagnosis and photodynamic therapy, 2019-12, Vol.28, p.58-64 [Periódico revisado por pares]

Netherlands: Elsevier B.V

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  • Título:
    Photodynamic inactivation for in vitro decontamination of Staphylococcus aureus in whole blood
  • Autor: Corrêa, Thaila Quatrini ; Blanco, Kate Cristina ; Soares, Jennifer Machado ; Inada, Natalia Mayumi ; Kurachi, Cristina ; Golim, Marjorie de Assis ; Deffune, Elenice ; Bagnato, Vanderlei Salvador
  • Assuntos: Bacteremia - drug therapy ; Blood ; Blood-Borne Pathogens - drug effects ; Blood-Borne Pathogens - radiation effects ; Decontamination ; Decontamination - methods ; Hematoporphyrins - pharmacology ; Humans ; In Vitro Techniques ; Photochemotherapy - methods ; Photodynamic inactivation ; Photosensitizing Agents - pharmacology ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - radiation effects
  • É parte de: Photodiagnosis and photodynamic therapy, 2019-12, Vol.28, p.58-64
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: •S. aureus photoinactivation pattern was different in media with blood derivatives.•Plasma appears to be the component responsible for hindering bacteria inactivation.•Photodynamic inactivation showed lower erythrocytes hemolysis when in whole blood.•Plasma is fundamental to the hemolysis process occur in a smaller percentage.•S. aureus inactivation in whole blood resulted in better outcomes. Blood can be the target of microbial cells in the human body. Erythrocytes, platelets, and plasma concentrates in blood bags used in hemotherapy for blood transfusion are contamination targets, which can trigger serious diseases in blood. These infections can cause septicemia that can lead to death if not recognized rapidly and treated adequately. The aim of this study was to evaluate the photodynamic inactivation in the in vitro decontamination of Staphylococcus aureus in whole blood, erythrocytes and platelet-rich plasma. Photodynamic inactivation using light doses of 10, 15 and 30 J/cm2 at 630 nm and an hematoporphyrin-derivative photosensitizer (Photogem®) solutions at 25 and 50 μg/mL were evaluated. Toxicity of treatment was determined by hemolysis and cell viability assays. Results: The S. aureus reduction in phosphate buffered saline (PBS), whole blood, erythrocytes and platelet-rich plasma at 15 J/cm2 and 50 μg/mL were 7.2, 1.0, 1.3 and 0.4 log CFU/mL, respectively. Quantitative and qualitative analyses were performed in whole blood samples, and Photogem® showed a low risk of hemolysis (10.7%) in whole blood. However, 100% of erythrocytes suffered hemolysis in the absence of plasma. The cell viability assay showed 13.9% of apoptosis in erythrocytes, but normal platelet viability. S. aureus inactivation of whole blood samples using 50 μg/mL Photogem® and 15 J/cm2 resulted in better outcomes, providing promising indications for treatment of bacterial contamination of blood, and in this work, alternative possibilities to apply the technique for blood decontamination are discussed.
  • Editor: Netherlands: Elsevier B.V
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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