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Comparative analysis of human gut microbiota by barcoded pyrosequencing

Andersson, Anders F ; Lindberg, Mathilda ; Jakobsson, Hedvig ; Bäckhed, Fredrik ; Nyrén, Pål ; Engstrand, Lars Ahmed, Niyaz

PloS one, 2008-07, Vol.3 (7), p.e2836-e2836 [Peer Reviewed Journal]

United States: Public Library of Science

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Title:
Comparative analysis of human gut microbiota by barcoded pyrosequencing
Author: Andersson, Anders F ; Lindberg, Mathilda ; Jakobsson, Hedvig ; Bäckhed, Fredrik ; Nyrén, Pål ; Engstrand, Lars
Ahmed, Niyaz
Subjects: Acids ; Adult ; Aged ; Analysis ; Bacteria ; Bar codes ; Biologi ; Biological ; Biology ; Biopsy ; Biotechnology/Applied Microbiology ; Cloning ; Communities ; Comparative analysis ; Disease control ; DNA ; DNA barcoding ; DNA Primers ; DNA sequencing ; Ecology ; Endoscopy ; Gastroenterology and Hepatology/Gastrointestinal Infections ; Gene sequencing ; Genetic Techniques ; Genetics and Genomics/Microbial Evolution and Genomics ; Health aspects ; Helicobacter pylori ; Humans ; Infectious diseases ; Infectious Diseases/Gastrointestinal Infections ; Intestinal microflora ; Intestines - microbiology ; MEDICIN ; Medicin och hälsovetenskap ; MEDICINE ; Methods ; Microbial activity ; Microbiological Techniques ; Microbiology in the medical area ; Microbiology/Applied Microbiology ; Microbiology/Environmental Microbiology ; Microbiology/Medical Microbiology ; Microbiota (Symbiotic organisms) ; Microorganisms ; Middle Aged ; Mikrobiologi inom det medicinska området ; Models, Biological ; Molecular Biology/Bioinformatics ; NATURAL SCIENCES ; NATURVETENSKAP ; Nucleotide sequencing ; Pharynx ; Phylogenetics ; Phylogeny ; Population ; RNA ; rRNA 16S ; Sequence Analysis ; Sequence Analysis, DNA ; Stomach ; Stomach - microbiology ; Taxonomy ; Ulcers ; Variable region
Is Part Of: PloS one, 2008-07, Vol.3 (7), p.e2836-e2836
Notes: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Conceived and designed the experiments: AFA ML HJ PN LE. Performed the experiments: AFA ML HJ. Analyzed the data: AFA ML HJ FB LE. Contributed reagents/materials/analysis tools: AFA PN LE. Wrote the paper: AFA ML HJ FB PN LE.
Description: Humans host complex microbial communities believed to contribute to health maintenance and, when in imbalance, to the development of diseases. Determining the microbial composition in patients and healthy controls may thus provide novel therapeutic targets. For this purpose, high-throughput, cost-effective methods for microbiota characterization are needed. We have employed 454-pyrosequencing of a hyper-variable region of the 16S rRNA gene in combination with sample-specific barcode sequences which enables parallel in-depth analysis of hundreds of samples with limited sample processing. In silico modeling demonstrated that the method correctly describes microbial communities down to phylotypes below the genus level. Here we applied the technique to analyze microbial communities in throat, stomach and fecal samples. Our results demonstrate the applicability of barcoded pyrosequencing as a high-throughput method for comparative microbial ecology.
Publisher: United States: Public Library of Science
Language: English

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Comparative analysis of human gut microbiota by barcoded pyrosequencing

Andersson, Anders F ; Lindberg, Mathilda ; Jakobsson, Hedvig ; Bäckhed, Fredrik ; Nyrén, Pål ; Engstrand, Lars Ahmed, Niyaz

United States: Public Library of Science

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