Idioma:

Influence of the 5'-untranslated region of ornithine decarboxylase mRNA and spermidine on ornithine decarboxylase synthesis

ITO, K ; KASHIWAGI, K ; WATANABE, S.-I ; KAMEJI, T ; HAYASHI, S.-I ; IGARASHI, K

The Journal of biological chemistry, 1990-08, Vol.265 (22), p.13036-13041 [Revista revisada por pares]

Bethesda, MD: American Society for Biochemistry and Molecular Biology

Texto completo disponible

Citas Citado por

Acciones

Título:
Influence of the 5'-untranslated region of ornithine decarboxylase mRNA and spermidine on ornithine decarboxylase synthesis
Autor: ITO, K ; KASHIWAGI, K ; WATANABE, S.-I ; KAMEJI, T ; HAYASHI, S.-I ; IGARASHI, K
Materias: Analytical, structural and metabolic biochemistry ; Animals ; Base Sequence ; Biological and medical sciences ; Cell-Free System ; DNA - genetics ; Enzymes and enzyme inhibitors ; Fundamental and applied biological sciences. Psychology ; Ligases ; Mice ; Molecular Sequence Data ; Ornithine Decarboxylase - biosynthesis ; Ornithine Decarboxylase - genetics ; Plasmids ; Protein Biosynthesis ; Rabbits ; Restriction Mapping ; Reticulocytes - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Spermidine - pharmacology
Es parte de: The Journal of biological chemistry, 1990-08, Vol.265 (22), p.13036-13041
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
Descripción: The effect of the 5'-untranslated region of ornithine decarboxylase (ODC) mRNA and of spermidine on the translation of ODC mRNA was studied in a rabbit reticulocyte cell-free system. The ODC mRNAs, possessing different sizes of the 5'-untranslated region and named mODC241, mODC188, mODC85, and mODC24 according to the number of nucleotides in the 5'-untranslated region, were synthesized by in vitro transcription techniques through the use of plasmids containing various sizes of mouse ODC cDNA. As the size of the 5'-untranslated region of ODC mRNA increased, the efficiency of the translation decreased and the degree of stimulation of the translation by 0.2 mM spermidine increased. The inhibition of ODC mRNA translation by high concentrations of spermidine (0.6-1 mM) also increased with an increase in the size of the 5'-untranslated region. The spermidine effects were increased greatly when the size of the 5'-untranslated region of ODC mRNA was increased from 85 to 188 nucleotides. These results suggest that the nucleotides 70-220 upstream from the initiator AUG are involved in the decrease in translational efficiency. In addition, at least the nucleotides in the 70-170 region upstream from the initiator AUG are important in the strong stimulation of ODC synthesis by low spermidine concentrations and in the inhibition of ODC synthesis at high spermidine concentrations.
Editor: Bethesda, MD: American Society for Biochemistry and Molecular Biology
Idioma: Inglés

Enlaces

Volver a la lista de resultados

Anterior Resultado 7 Siguiente Ir a la siguiente página

Realización: Logos de Redes Sociales:

Influence of the 5'-untranslated region of ornithine decarboxylase mRNA and spermidine on ornithine decarboxylase synthesis

ITO, K ; KASHIWAGI, K ; WATANABE, S.-I ; KAMEJI, T ; HAYASHI, S.-I ; IGARASHI, K

Bethesda, MD: American Society for Biochemistry and Molecular Biology

Buscando en bases de datos remotas, por favor espere