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Importance of the Cyanobacterial Gun4 Protein for Chlorophyll Metabolism and Assembly of Photosynthetic Complexes

Sobotka, Roman ; Dühring, Ulf ; Komenda, Josef ; Peter, Enrico ; Gardian, Zdenko ; Tichy, Martin ; Grimm, Bernhard ; Wilde, Annegret

The Journal of biological chemistry, 2008-09, Vol.283 (38), p.25794-25802 [Periódico revisado por pares]

United States: Elsevier Inc

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Título:
Importance of the Cyanobacterial Gun4 Protein for Chlorophyll Metabolism and Assembly of Photosynthetic Complexes
Autor: Sobotka, Roman ; Dühring, Ulf ; Komenda, Josef ; Peter, Enrico ; Gardian, Zdenko ; Tichy, Martin ; Grimm, Bernhard ; Wilde, Annegret
Assuntos: Carrier Proteins - genetics ; Carrier Proteins - physiology ; Cell Membrane - metabolism ; Chlorophyll - chemistry ; Chlorophyll - metabolism ; Chloroplasts - metabolism ; Cyanobacteria - metabolism ; Intracellular Signaling Peptides and Proteins - genetics ; Intracellular Signaling Peptides and Proteins - metabolism ; Intracellular Signaling Peptides and Proteins - physiology ; Light-Harvesting Protein Complexes - metabolism ; Lyases - chemistry ; Metabolism and Bioenergetics ; Microscopy, Electron ; Microscopy, Electron, Transmission ; Mutation ; Phenotype ; Photosynthesis ; Photosynthetic Reaction Center Complex Proteins - chemistry ; Photosynthetic Reaction Center Complex Proteins - physiology ; Photosystem II Protein Complex - metabolism ; Porphyrins - chemistry ; Spectrometry, Fluorescence - methods
É parte de: The Journal of biological chemistry, 2008-09, Vol.283 (38), p.25794-25802
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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These authors equally contributed to this work.
This work was supported by Deutsche Forschungsgemeinschaft Grants SFB429 and TPA8 (to A. W. and B. G.), by Institutional Research Concept AV0Z50200510, by Ministry of Education of the Czech Republic project MSM6007665808 (to J. K.), and by Grant Agency of the Czech Academy of Sciences project IAA500200713 (to R. S. and M. T.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Descrição: Gun4 is a porphyrin-binding protein that activates magnesium chelatase, a multimeric enzyme catalyzing the first committed step in chlorophyll biosynthesis. In plants, GUN4 has been implicated in plastid-to-nucleus retrograde signaling processes that coordinate both photosystem II and photosystem I nuclear gene expression with chloroplast function. In this work we present the functional analysis of Gun4 from the cyanobacterium Synechocystis sp. PCC 6803. Affinity co-purification of the FLAG-tagged Gun4 with the ChlH subunit of the magnesium chelatase confirmed the association of Gun4 with the enzyme in cyanobacteria. Inactivation of the gun4 gene abolished photoautotrophic growth of the resulting gun4 mutant strain that exhibited a decreased activity of magnesium chelatase. Consequently, the cellular content of chlorophyll-binding proteins was highly inadequate, especially that of proteins of photosystem II. Immunoblot analyses, blue native polyacrylamide gel electrophoresis, and radiolabeling of the membrane protein complexes suggested that the availability of the photosystem II antenna protein CP47 is a limiting factor for the photosystem II assembly in the gun4 mutant.
Editor: United States: Elsevier Inc
Idioma: Inglês

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Importance of the Cyanobacterial Gun4 Protein for Chlorophyll Metabolism and Assembly of Photosynthetic Complexes

Sobotka, Roman ; Dühring, Ulf ; Komenda, Josef ; Peter, Enrico ; Gardian, Zdenko ; Tichy, Martin ; Grimm, Bernhard ; Wilde, Annegret

United States: Elsevier Inc

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