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Amphibians on the hotspot: Molecular biology and conservation in the South American Atlantic Rainforest

Amaral, Cesar R L ; Chaves, Anna C S ; Borges Júnior, Vitor N T ; Pereira, Filipe ; Silva, Bruna M ; Silva, Dayse A ; Amorim, António ; Carvalho, Elizeu F ; Rocha, Carlos F D Abellán, Pedro

PloS one, 2019-10, Vol.14 (10), p.e0224320 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Amphibians on the hotspot: Molecular biology and conservation in the South American Atlantic Rainforest
Autor: Amaral, Cesar R L ; Chaves, Anna C S ; Borges Júnior, Vitor N T ; Pereira, Filipe ; Silva, Bruna M ; Silva, Dayse A ; Amorim, António ; Carvalho, Elizeu F ; Rocha, Carlos F D
Abellán, Pedro
Assuntos: Amphibia ; Amphibians ; Amphibians - genetics ; Animal models ; Animals ; Atlantic Ocean ; Biodiversity ; Biology and Life Sciences ; Computer and information sciences ; Conservation ; Conservation of Natural Resources ; Deoxyribonucleic acid ; DNA ; DNA barcoding ; Domains ; Ecology ; Ecology and Environmental Sciences ; Ecosystem biology ; Ecosystem services ; Ecosystems ; Evolution ; Food chains ; Food sources ; Food webs ; Frogs ; Gene sequencing ; Genomes ; Identification ; Larva - genetics ; Maintenance ; Mitochondria ; Molecular Biology ; Nutrient cycles ; Polymerase chain reaction ; Population decline ; Rainforest ; Rainforests ; Regulators ; Reptiles & amphibians ; Research and analysis methods ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; South America ; Species diversity ; Vertebrates
É parte de: PloS one, 2019-10, Vol.14 (10), p.e0224320
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Competing Interests: The authors have declared that no competing interests exist.
Descrição: Amphibians are the focus of a recent debate and public attention owing to the global decline in their populations worldwide. Amphibians are one of the most threatened and poorly known groups of vertebrates in several geographic areas, even though they play a central role in their own ecosystems. At different levels, amphibians make their contribution to proper ecosystem functioning. They act as regulators of the food web and nutrient cycling, and they also provide several valuable ecosystem services, e.g., as a food source and as animal models for lab research. In this sense, it seems clear that the maintenance of amphibian diversity should be one of the major goals for the several countries where their population decline is observed. However, we are still struggling with the very first step of this process, i.e., the correct identification of the amphibian species diversity. Over the past few decades, research on molecular identification of amphibians using DNA barcoding has encountered some difficulties related to high variability in the mitochondrial genome of amphibians, and a research gap is noticeable in the literature. We herein evaluated both COI and 16S rRNA mitochondrial genes for the molecular identification of frogs and tadpoles in a large fragment of the South American Atlantic Rainforest in Rio de Janeiro, Brazil. Our results suggest that both COI and 16S rRNA are informative markers for the molecular identification of the amphibian specimens with all specimens unambiguously identified at the species level. We also made publicly available 12 new sequences of Atlantic Rainforest amphibian species for the first time, and we discussed some conservation issues related to amphibians within the Atlantic Rainforest domains in the state of Rio de Janeiro, Brazil.
Editor: United States: Public Library of Science
Idioma: Inglês

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Amphibians on the hotspot: Molecular biology and conservation in the South American Atlantic Rainforest

Amaral, Cesar R L ; Chaves, Anna C S ; Borges Júnior, Vitor N T ; Pereira, Filipe ; Silva, Bruna M ; Silva, Dayse A ; Amorim, António ; Carvalho, Elizeu F ; Rocha, Carlos F D Abellán, Pedro

United States: Public Library of Science

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