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Cementocyte alterations associated with experimentally induced cellular cementum apposition in Hyp mice

Lira dos Santos, Elis J. ; Salmon, Cristiane R. ; Chavez, Michael B. ; Almeida, Amanda B. ; Tan, Michelle H. ; Chu, Emily Y. ; Sallum, Enilson A. ; Casati, Marcio Z. ; Ruiz, Karina G. S. ; Kantovitz, Kamila R. ; Foster, Brian L. ; Nociti Júnior, Francisco H.

Journal of periodontology (1970), 2021-11, Vol.92 (11), p.116-127 [Revista revisada por pares]

United States

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  • Título:
    Cementocyte alterations associated with experimentally induced cellular cementum apposition in Hyp mice
  • Autor: Lira dos Santos, Elis J. ; Salmon, Cristiane R. ; Chavez, Michael B. ; Almeida, Amanda B. ; Tan, Michelle H. ; Chu, Emily Y. ; Sallum, Enilson A. ; Casati, Marcio Z. ; Ruiz, Karina G. S. ; Kantovitz, Kamila R. ; Foster, Brian L. ; Nociti Júnior, Francisco H.
  • Materias: Animals ; bone ; Dental Cementum ; extracellular matrix ; hypophosphatemic rickets ; Mice ; Molar ; periodontium ; Tooth ; tooth eruption ; Tooth Root - diagnostic imaging ; X-Ray Microtomography
  • Es parte de: Journal of periodontology (1970), 2021-11, Vol.92 (11), p.116-127
  • Notas: Elis J. Lira dos Santos and Cristiane R. Salmon contributed equally to this study.
    Brian L. Foster and Francisco H. Nociti Júnior contributed equally as mentors for this study.
    Authors contributing equally to this article
    AUTHOR CONTRIBUTIONS
    E.J. Lira do Santos contributed to conception, design, data acquisition, analysis, and interpretation, drafted and critically revised the manuscript; C.R. Salmon contributed to conception, design, data acquisition, analysis, and interpretation, drafted and critically revised the manuscript; M.B. Chavez contributed to conception, design, data acquisition, analysis, and interpretation, drafted and critically revised the manuscript; A. Bandeira de Almeida contributed to data acquisition and critically revised the manuscript; M.H. Tan contributed to data acquisition and analysis, and critically revised the manuscript; E.Y. Chu contributed to design, data acquisition, analysis, and interpretation, and critically revised the manuscript; E.A. Sallum, M.Z. Casati contributed to design, interpretation and critically revised the manuscript; K.G. Ruiz contributed to design, analysis, and interpretation and critically revised the manuscript ; K.R. Kantovitz contributed to data acquisition and analysis, and critically revised the manuscript; B.L. Foster contributed to conception, design, data acquisition, analysis, and interpretation, drafted and critically revised the manuscript. F.H. Nociti, Jr. contributed to conception, design, data acquisition, analysis, and interpretation, drafted and critically revised the manuscript. All authors gave final approval and agree to be accountable for all aspects of the work.
    Authors contributing equally as mentors for this article
  • Descripción: Background Cellular cementum, a mineralized tissue covering apical tooth roots, grows by apposition to maintain the tooth in its occlusal position. We hypothesized that resident cementocytes would show morphological changes in response to cementum apposition, possibly implicating a role in cementum biology. Methods Mandibular first molars were induced to super‐erupt (EIA) by extraction of maxillary molars, promoting rapid new cementum formation. Tissue and cell responses were analyzed at 6 and/or 21 days post‐procedure (dpp). Results High‐resolution micro‐computed tomography (micro‐CT) and confocal laser scanning microscopy showed increased cellular cementum by 21 dpp. Transmission electron microscopy (TEM) revealed that cementocytes under EIA were 50% larger than control cells, supported by larger pore sizes detected by micro‐CT. Cementocytes under EIA displayed ultrastructural changes consistent with increased activity, including increased cytoplasm and nuclear size. We applied EIA to Hyp mutant mice, where cementocytes have perilacunar hypomineralization defects, to test cell and tissue responses in an altered mechanoresponsive milieu. Hyp and WT molars displayed similar super‐eruption, with Hyp molars exhibiting 28% increased cellular cementum area versus 22% in WT mice at 21 dpp. Compared to control, Hyp cementocytes featured well‐defined, disperse euchromatin and a thick layer of peripherally condensed heterochromatin in nuclei, indicating cellular activity. Immunohistochemistry (IHC) for cementum markers revealed intense dentin matrix protein‐1 expression and abnormal osteopontin deposition in Hyp mice. Both WT and Hyp cementocytes expressed gap junction protein, connexin 43. Conclusion This study provides new insights into the EIA model and cementocyte activity in association with new cementum formation.
  • Editor: United States
  • Idioma: Inglés
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