skip to main content
Idioma:

Characterization of potentially probiotic lactic acid bacteria and bifidobacteria isolated from human colostrum

Liu, Wenjun ; Chen, Meixuan ; Duo, Lana ; Wang, Jicheng ; Guo, Shuai ; Sun, Haotian ; Menghe, Bilige ; Zhang, Heping

Journal of dairy science, 2020-05, Vol.103 (5), p.4013-4025 [Periódico revisado por pares]

United States: Elsevier Inc

Texto completo disponível

Citações Citado por
  • Título:
    Characterization of potentially probiotic lactic acid bacteria and bifidobacteria isolated from human colostrum
  • Autor: Liu, Wenjun ; Chen, Meixuan ; Duo, Lana ; Wang, Jicheng ; Guo, Shuai ; Sun, Haotian ; Menghe, Bilige ; Zhang, Heping
  • Assuntos: artificial gastric juice ; bile salt ; human colostrum ; isolation/identification ; lactic acid bacteria
  • É parte de: Journal of dairy science, 2020-05, Vol.103 (5), p.4013-4025
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: Breast milk is the main source of nutrition for infants; it contains considerable microflora that can be transmitted to the infant endogenously or by breastfeeding, and it plays an important role in the maturation and development of the immune system. In this study, we isolated and identified lactic acid bacteria (LAB) from human colostrum, and screened 2 strains with probiotic potential. The LAB isolated from 40 human colostrum samples belonged to 5 genera: Lactobacillus, Bifidobacterium, Streptococcus, Enterococcus, and Staphylococcus. We also isolated Propionibacterium and Actinomyces. We identified a total of 197 strains of LAB derived from human colostrum based on their morphology and 16S rRNA sequence, among them 8 strains of Bifidobacterium and 10 strains of Lactobacillus, including 3 Bifidobacterium species and 4 Lactobacillus species. The physiological and biochemical characteristics of strains with good probiotic characteristics were evaluated. The tolerances of some of the Bifidobacterium and Lactobacillus strains to gastrointestinal fluid and bile salts were evaluated in vitro, using the probiotic strains Bifidobacterium lactis BB12 and Lactobacillus rhamnosus GG as controls. Among them, B. lactis Probio-M8 and L. rhamnosus Probio-M9 showed survival rates of 97.25 and 78.33% after digestion for 11 h in artificial gastrointestinal juice, and they exhibited growth delays of 0.95 and 1.87 h, respectively, in 0.3% bile salts. These two strains have the potential for application as probiotics and will facilitate functional studies of probiotics in breast milk and the development of human milk-derived probiotics.
  • Editor: United States: Elsevier Inc
  • Idioma: Inglês
Links
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.