Idioma:

Normalization of multiple RNA samples using an in vitro-synthesized external standard cRNA

Toscani, Antonio ; Soprano, Dianne Robert ; Cosenza, Stephen C. ; Owen, Thomas A. ; Soprano, Kenneth J.

Analytical biochemistry, 1987-09, Vol.165 (2), p.309-319 [Periódico revisado por pares]

San Diego, CA: Elsevier Inc

Texto completo disponível

Citações Citado por

Enviar para

Título:
Normalization of multiple RNA samples using an in vitro-synthesized external standard cRNA
Autor: Toscani, Antonio ; Soprano, Dianne Robert ; Cosenza, Stephen C. ; Owen, Thomas A. ; Soprano, Kenneth J.
Assuntos: Animals ; Biological and medical sciences ; Cells, Cultured ; Cloning, Molecular - methods ; Diverse techniques ; DNA - analysis ; Fundamental and applied biological sciences. Psychology ; in vitro transcription ; Molecular and cellular biology ; Northern blotting ; Plasmids ; RNA - analysis ; RNA - chemical synthesis ; RNA - genetics ; RNA, Complementary ; RNA, Messenger - genetics ; RNase protection
É parte de: Analytical biochemistry, 1987-09, Vol.165 (2), p.309-319
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: A method for the normalization of multiple RNA samples is described. This method exploits the recently developed technology which allows the synthesis of single-stranded, specific RNA molecules in vitro using either SP6 or T7 RNA polymerase to prepare an external standard cRNA. When this external standard cRNA is added to cell samples at the time of lysis, it becomes a stable, integral part of the RNA content of each sample, which can easily and reproducibly be detected and quantitated by either Northern blot or RNase protection. The feasibility of this approach to normalization has been tested in a mouse 3T3 cell model system. In multiple samples, the relative levels of this externally added standard transcript are shown to closely parallel the relative levels of an internal standard control transcript as well as the amount of RNA determined by spectrophotometric analysis. The data obtained demonstrate that an externally added, in vitro-synthesized transcript can serve as an accurate, universal means of normalizing multiple RNA samples, since it is not dependent on sample RNA concentration, species, cell or tissue type, or experimental manipulation.
Editor: San Diego, CA: Elsevier Inc
Idioma: Inglês

Links

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Normalization of multiple RNA samples using an in vitro-synthesized external standard cRNA

Toscani, Antonio ; Soprano, Dianne Robert ; Cosenza, Stephen C. ; Owen, Thomas A. ; Soprano, Kenneth J.

San Diego, CA: Elsevier Inc

Buscando em bases de dados remotas. Favor aguardar.