skip to main content
Idioma:

Modelling Inherited Prion Diseases In Mice

Owusu-Yaw, Bernie Simone

UCL (University College London) 2022

Texto completo disponible

Citas Citado por
  • Título:
    Modelling Inherited Prion Diseases In Mice
  • Autor: Owusu-Yaw, Bernie Simone
  • Notas: Doctoral thesis, UCL (University College London).
  • Descripción: Prion diseases are fatal neurodegenerative diseases of mammals, caused by a misfolded infectious protein. There are 3 aetiological forms namely sporadic, acquired or inherited. The genetic component makes it possible for transgenic technology to be applied to the modelling of prion diseases. In 2013 Mead et al. reported a novel prion disease linked to a stop codon mutation at residue 163 (Y163X) in the human prion protein gene (PRNP). This mutation results in the production of a truncated prion protein (PrP) lacking both glycosylation sites and GPI anchor, and it is associated with an atypical prion disease phenotype including diarrhoea, autonomic neuropathy and the accumulation of PrP amyloid in peripheral organs and blood vessels. Typically, prion diseases predominantly affect the nervous system so the uniqueness of the Y163X disease phenotype makes it a good candidate for the study of prion therapeutics. The aim of this project was therefore to develop mouse models expressing human PrP Y163X that recapitulate human disease and then to study ageing cohorts of homozygous and heterozygous mice for spontaneous amyloid deposition. Two different approaches were pursued as follows: (1) Conventional transgenic approach (2) Gene targeted approach (CRISPR- Cas9 and knock-in technology). Six transgenic lines expressing HuPrP 163X under the control of the ubiquitous CAG promoter were generated alongside transgenic wild-type controls expressing wild-type human PrP. Y163X knock-in mice and control knock-in mice expressing wild-type human PrP generated from blastocyst injection of embryonic stem cells were made commercially. These lines were characterised for expression of the truncated 163X human prion protein using techniques such as western blotting, ELISA, fluorescent immunohistochemistry and RT-PCR. Aging cohorts from homozygous lines were set up in long term observation experiments. A panel of CNS and peripheral tissues has been isolated at defined time points for immunohistochemical analysis to determine if the expression of HuPrP Y163X in transgenic and knock-in mice is sufficient to cause spontaneous PrP amyloid deposition.
  • Editor: UCL (University College London)
  • Fecha de creación: 2022
  • Idioma: Inglés
Enlaces
Volver a la lista de resultados
Ir a la página anteriorAnterior Resultado  10 SiguienteIr a la siguiente página

  • Realización: ABCD-USP USP   Logos de Redes Sociales: Freepik

Buscando en bases de datos remotas, por favor espere

  • Buscando por
  • enscope:(USP_VIDEOS),scope:("PRIMO"),scope:(USP_FISICO),scope:(USP_EREVISTAS),scope:(USP),scope:(USP_EBOOKS),scope:(USP_PRODUCAO),primo_central_multiple_fe
  • Mostrar lo que tiene hasta ahora