In vitro chemosensitivity of head and neck cancer cell lines

• Autor: Schuler, P J ; Trellakis, S ; Greve, J ; Bas, M ; Bergmann, C ; Bölke, E ; Lehnerdt, G ; Mattheis, S ; Albers, A E ; Brandau, S ; Lang, S ; Whiteside, T L ; Bier, H ; Hoffmann, T K
• Assuntos: Analysis ; Antimitotic agents ; Antineoplastic agents ; Antineoplastic Agents - pharmacology ; Cancer ; Carboplatin - pharmacology ; Carcinoma, Squamous Cell - drug therapy ; Carcinoma, Squamous Cell - pathology ; Care and treatment ; Cell line ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Cells ; Chemosensitivity ; Chemotherapy ; Cisplatin - pharmacology ; Drug resistance ; Drug Resistance, Neoplasm ; Head and neck cancer ; Head and Neck Neoplasms - drug therapy ; Head and Neck Neoplasms - pathology ; Humans ; Methotrexate ; Methotrexate - pharmacology ; MTT ; Paclitaxel - pharmacology ; Squamous cell carcinoma ; Tumors
• É parte de: European journal of medical research, 2010-08, Vol.15 (8), p.337-337
• Descrição: Systemic treatment of head and neck squamous cell carcinoma (HNSCC) includes a variety of antineoplastic drugs. However, drug-resistance interferes with the effectiveness of chemotherapy. Preclinical testing models are needed in order to develop approaches to overcome chemoresistance. - Ten human cell lines were obtained from HNSCC, including one with experimentally-induced cisplatin resistance. Inhibition of cell growth by seven chemotherapeutic agents (cisplatin, carboplatin, 5- fluorouracil, methotrexate, bleomycin, vincristin, and paclitaxel) was measured using metabolic MTT-uptake assay and correlated to clinically-achievable plasma concentrations. - All drugs inhibited cell growth in a concentration-dependent manner with an IC50 comparable to that achievable in vivo. However, response curves for methotrexate were unsatisfactory and for paclitaxel, the solubilizer cremophor EL was toxic. Cross-resistance was observed between cisplatin and carboplatin. - Chemosensitivity of HNSCC cell lines can be determined using the MTT-uptake assay. For DNA-interfering cytostatics and vinca alkaloids this is a simple and reproducible procedure. Determined in vitro chemosensitivity serves as a baseline for further experimental approaches aiming to modulate chemoresistance in HNSCC with potential clinical significance.
  
• Editor: England: BioMed Central Ltd
  
• Idioma: Inglês