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Challenges in the identification of Ochrobactrum anthropi in blood and sputum cultures of patients with cystic fibrosis

Carlos Emílio Levy ; Fernando Augusto LIma Marson ; Élio Barreto Carvalho Filho

Revista de Epidemiologia e Controle de Infecção, 2018, Vol.8 (2), p.189-191 [Periódico revisado por pares]

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Título:
Challenges in the identification of Ochrobactrum anthropi in blood and sputum cultures of patients with cystic fibrosis
Autor: Carlos Emílio Levy ; Fernando Augusto LIma Marson ; Élio Barreto Carvalho Filho
É parte de: Revista de Epidemiologia e Controle de Infecção, 2018, Vol.8 (2), p.189-191
Descrição: Background and objectives: The Ochrobactrum anthropic identification is a challenge. In this context, we perform numerous methods to identify the O. anthropic and associated the achieved results among the methods. Methods: We performed the follow methods: manual phenotypic, Vitek®2, Phoenix™, polymerase chain reaction, MALDI-TOF Vitek MS™ and BioTyper™. Results: Biochemical tests showed limitations to identify O. anthropic. Vitek®2 was the only method that showed the same result seen in polymerase chain reaction for O. anthropic, in all samples, followed by Phoenix™. MALDI-MS™ failed at defining at species level, and MALDI-BD™ presented high diversity in identification, showing to be a test of low specificity. Conclusions: Each method showed advantages and disadvantages to identify the O. anthropic. Keywords: MALDI-TOF; PCR; Phoenix™; Ochrobactrum anthropic; Vitek®2
Editor: Universidade de Santa Cruz do Sul
Idioma: Português

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