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Development of quinoxaline 1, 4-dioxides resistance in Escherichia coli and molecular change under resistance selection

Guo, Wentao ; Hao, Haihong ; Dai, Menghong ; Wang, Yulian ; Huang, Lingli ; Peng, Dapeng ; Wang, Xu ; Wang, Hailan ; Yao, Min ; Sun, Yawei ; Liu, Zhenli ; Yuan, Zonghui Khan, Asad U.

PloS one, 2012-08, Vol.7 (8), p.e43322-e43322 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Development of quinoxaline 1, 4-dioxides resistance in Escherichia coli and molecular change under resistance selection
Autor: Guo, Wentao ; Hao, Haihong ; Dai, Menghong ; Wang, Yulian ; Huang, Lingli ; Peng, Dapeng ; Wang, Xu ; Wang, Hailan ; Yao, Min ; Sun, Yawei ; Liu, Zhenli ; Yuan, Zonghui
Khan, Asad U.
Assuntos: ABC transporters ; Animals ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Antimicrobial agents ; Bacteria ; Base Sequence ; Bills ; Biodegradation ; Biology ; Biosynthesis ; Clinical isolates ; Conjugation ; Dioxides ; DNA Primers - genetics ; DNA, Complementary - metabolism ; DNA-directed RNA polymerase ; Drug resistance in microorganisms ; Drug Resistance, Bacterial ; Drugs ; E coli ; Electron transport ; Electron transport chain ; Emergency vehicles ; Escherichia coli ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Escherichia coli Proteins - genetics ; Genes ; Genetic aspects ; Genetic regulation ; Glycolysis ; Growth promoters ; Horizontal transfer ; Hybridization ; Laboratories ; Lipid A ; Livestock ; Medicine ; Metabolism ; Microbial drug resistance ; Microbial Sensitivity Tests - methods ; Minimum inhibitory concentration ; Models, Genetic ; Molecular Sequence Data ; Nucleic Acid Hybridization ; Oxidative phosphorylation ; Oxides - pharmacology ; Phenotype ; Phosphorylation ; Physiological aspects ; Polymerase Chain Reaction - methods ; Protein biosynthesis ; Proteins ; Quinoxaline ; Quinoxalines - chemistry ; Quinoxalines - pharmacology ; Salmonella ; Strains (organisms) ; Subtraction ; Swine ; Transcription ; Trehalose ; Veterinary Science
É parte de: PloS one, 2012-08, Vol.7 (8), p.e43322-e43322
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Conceived and designed the experiments: WG MD HH XW ZY. Performed the experiments: WG HW MY YS. Analyzed the data: WG HH MD DP ZY. Contributed reagents/materials/analysis tools: ZY YW LH ZL. Wrote the paper: WG HH MD ZY.
Competing Interests: The authors have declared that no competing interests exist.
Descrição: Quinoxaline 1, 4-dioxides (QdNOs) has been used in animals as antimicrobial agents and growth promoters for decades. However, the resistance to QdNOs in pathogenic bacteria raises worldwide concern but it is barely known. To explore the molecular mechanism involved in development of QdNOs resistance in Escherichia coli, 6 strains selected by QdNOs in vitro and 21 strains isolated from QdNOs-used swine farm were subjected to MIC determination and PCR amplification of oqxA gene. A conjugative transfer was carried out to evaluate the transfer risk of QdNOs resistant determinant. Furthermore, the transcriptional profile of a QdNOs-resistant E. coli (79O4-2) selected in vitro with its parent strain 79-161 was assayed with a prokaryotic suppression subtractive hybridization (SSH) PCR cDNA subtraction. The result showed that more than 95% (20/21) clinical isolates were oqxA positive, while all the 6 induced QdNOs-resistant strains carried no oqxA gene and exhibited low frequency of conjugation. 44 fragments were identified by SSH PCR subtraction in the QdNOs-resistant strain 79O4-2. 18 cDNAs were involved in biosynthesis of Fe-S cluster (narH), protein (rpoA, trmD, truA, glyS, ileS, rplFCX, rpsH, fusA), lipoate (lipA), lipid A (lpxC), trehalose (otsA), CTP(pyrG) and others molecular. The 11 cDNAs were related to metabolism or degradation of glycolysis (gpmA and pgi) and proteins (clpX, clpA, pepN and fkpB). The atpADG and ubiB genes were associated with ATP biosynthesis and electron transport chain. The pathway of the functional genes revealed that E. coli may adapt the stress generated by QdNOs or develop specific QdNOs-resistance by activation of antioxidative agents biosynthesis (lipoate and trehalose), protein biosynthesis, glycolysis and oxidative phosphorylation. This study initially reveals the possible molecular mechanism involved in the development of QdNOs-resistance in E. coli, providing with novel insights in prediction and assessment of the emergency and horizontal transfer of QdNOs-resistance in E. coli.
Editor: United States: Public Library of Science
Idioma: Inglês

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Development of quinoxaline 1, 4-dioxides resistance in Escherichia coli and molecular change under resistance selection

Guo, Wentao ; Hao, Haihong ; Dai, Menghong ; Wang, Yulian ; Huang, Lingli ; Peng, Dapeng ; Wang, Xu ; Wang, Hailan ; Yao, Min ; Sun, Yawei ; Liu, Zhenli ; Yuan, Zonghui Khan, Asad U.

United States: Public Library of Science

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