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Peptidorhamnomannan from Lomentospora prolificans modulates the inflammatory response in macrophages infected with Candida albicans

Xisto, Mariana Ingrid Dutra da Silva ; Santos, Suelen S ; Rossato, Luana ; Yoshikawa, Fábio Seiti Yamada ; Haido, Rosa Maria Tavares ; de Almeida, Sandro Rogério ; Barreto-Bergter, Eliana

BMC microbiology, 2020-08, Vol.20 (1), p.245-245, Article 245 [Periódico revisado por pares]

England: BioMed Central Ltd

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  • Título:
    Peptidorhamnomannan from Lomentospora prolificans modulates the inflammatory response in macrophages infected with Candida albicans
  • Autor: Xisto, Mariana Ingrid Dutra da Silva ; Santos, Suelen S ; Rossato, Luana ; Yoshikawa, Fábio Seiti Yamada ; Haido, Rosa Maria Tavares ; de Almeida, Sandro Rogério ; Barreto-Bergter, Eliana
  • Assuntos: Candida albicans ; Cell surface ; Cell survival ; Cell walls ; Chromatography ; Climate ; Conidia ; Cytokines ; Evaluation ; Fungi ; Fungicidal activity ; Fungicides ; Immune serum ; Immune system ; Infections ; Inflammation ; Inflammatory response ; Lomentospora prolificans ; Macrophages ; Mycelia ; Mycoses ; N-glycans ; Oligosaccharides ; Pathogens ; Peptidorhamnomannan ; Polysaccharides ; Tumor necrosis factor-α
  • É parte de: BMC microbiology, 2020-08, Vol.20 (1), p.245-245, Article 245
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: Peptidorhamnomannan is a glycoconjugate that consists of a peptide chain substituted by O- and N-linked glycans, present on the cell surface of Lomentospora prolificans, a saprophytic fungus which is widely distributed in regions with temperate climates. O-linked oligosaccharides from peptidorhamnomannan isolated from Lomentospora prolificans conidia are recognized by macrophages mediating macrophage - conidia interaction. In this work, peptidorhamnomannan was isolated from L. prolificans mycelium cell wall and its role in macrophage - Candida albicans interaction was evaluated. Purified peptidorhamnomannan inhibits the reactivity of rabbit immune sera to mycelial and conidia forms of L. prolificans, indicating that this glycoconjugate is exposed on the fungal surface and can mediate interaction with host immune cells. We demonstrated that peptidorhamnomannan leads to TNF-α production in J774 macrophages for 1, 2 and 3 h of incubation, suggesting that this glycoconjugate may have a beneficial role in the response to fungal infections. In order to confirm this possibility, the effect of peptidorhamnomannan on the macrophage - C. albicans interaction was evaluated. Macrophages treated with peptidorhamnomannan led to a lower fungal survival, suggesting that peptidorhamnomannan induces an increased fungicidal activity in macrophages. Furthermore, TNF-α levels were measured in supernatants after macrophage - C. albicans interaction for 1, 2 and 3 h. Peptidorhamnomannan treatment led to a higher TNF-α production at the beginning of the interaction. However, the release of TNF-α was not maintained after 1 h of incubation. Besides, peptidorhamnomannan did not show any inhibitory or fungicidal effect in C. albicans when used at 100 μg/ml but it was able to kill C. albicans at a concentration of 400 μg/ml. We suggest that peptidorhamnomannan acts as a molecular pattern on the invading pathogen, promotes TNF-α production and, thus, increases macrophage fungicidal activity against Candida albicans.
  • Editor: England: BioMed Central Ltd
  • Idioma: Inglês
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