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A rapid absorbance-based growth assay to screen the toxicity of oligomer Aβ42 and protect against cell death in yeast

Bharadwaj, Prashant ; Martins, Ralph

Neural regeneration research, 2020-10, Vol.15 (10), p.1931-1936 [Periódico revisado por pares]

Mumbai: Wolters Kluwer India Pvt. Ltd

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  • Título:
    A rapid absorbance-based growth assay to screen the toxicity of oligomer Aβ42 and protect against cell death in yeast
  • Autor: Bharadwaj, Prashant ; Martins, Ralph
  • Assuntos: alzheimer’s disease; amyloid toxicity; autophagy; aβ42 oligomer; high-throughput screening; latrepirdine; neuroprotection; yeast model ; Apoptosis ; Autophagy ; Brain ; Cell death ; Experiments ; Insulin ; Nitrogen ; Peptides ; Proteins ; Yeast
  • É parte de: Neural regeneration research, 2020-10, Vol.15 (10), p.1931-1936
  • Notas: Author contributions: Conceptualization, methodology, data acquisition, original draft preparation: PB; writing and review and funding acquisition: RM, PB. Both authors approved the final version of the paper.
  • Descrição: Multiple lines of evidence show that soluble oligomer forms of amyloid β protein (Aβ42) are the most neurotoxic species in the brain and correlates with the degree of neuronal loss and cognitive deficit in Alzheimer's disease. Although many studies have used mammalian cells to investigate oligomer Aβ42 toxicity, the use of more simple eukaryotic cellular systems offers advantages for large-scale screening studies. We have previously established and validated budding yeast, Saccharomyces cerevisiae to be a simple and a robust model to study the toxicity of Aβ. Using colony counting based methods, oligomeric Aβ42 was shown to induce dose-dependent cell death in yeast. We have adapted this method for high throughput screening by developing an absorbance-based growth assay. We further validated the assay with treatments previously shown to protect oligomer Aβ42 induced cell death in mammalian and yeast cells. This assay offers a platform for studying underlying mechanisms of oligomer Aβ42 induced cell death using gene deletion/overexpression libraries and developing novel agents that alleviate Aβ42 induced cell death.
  • Editor: Mumbai: Wolters Kluwer India Pvt. Ltd
  • Idioma: Inglês;Chinês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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