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High frequency gene transfer by microprojectile bombardment of intact conidia from the entomopathogenic fungus Paecilomyces fumosoroseus

Chaves Barreto, Cristine ; Cardoso Alves, Luciano ; Lima Aragão, Francisco José ; Rech, Elıćbio ; Schrank, Augusto ; Henning Vainstein, Marilene

FEMS microbiology letters, 1997-11, Vol.156 (1), p.95-99 [Periódico revisado por pares]

Oxford, UK: Blackwell Publishing Ltd

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Título:
High frequency gene transfer by microprojectile bombardment of intact conidia from the entomopathogenic fungus Paecilomyces fumosoroseus
Autor: Chaves Barreto, Cristine ; Cardoso Alves, Luciano ; Lima Aragão, Francisco José ; Rech, Elıćbio ; Schrank, Augusto ; Henning Vainstein, Marilene
Assuntos: Animals ; Anti-Bacterial Agents - pharmacology ; Biolistic ; Bombardment ; Conidia ; Deoxyribonucleic acid ; DNA ; DNA, Fungal - administration & dosage ; DNA, Fungal - genetics ; Drug Resistance, Microbial - genetics ; Entomopathogenic fungi ; Evaluation Studies as Topic ; Fungal transformation ; Gene transfer ; Gene Transfer Techniques ; Genetic Markers ; Hygromycin ; Hygromycin B - pharmacology ; Hygromycin B resistance ; Insecta - microbiology ; Microbiology ; Microprojectile bombardment ; Paecilomyces - drug effects ; Paecilomyces - genetics ; Paecilomyces - pathogenicity ; Paecilomyces fumosoroseus ; Pest Control, Biological ; Polyethylene Glycols ; Protoplasts ; Protoplasts - drug effects ; Transformation, Genetic
É parte de: FEMS microbiology letters, 1997-11, Vol.156 (1), p.95-99
Notas: ObjectType-Article-2
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Descrição: Abstract Two different methods, (i) PEG and (ii) biolistic, were employed to transform protoplasts and conidia of Paecilomyces fumosoroseus using hygromycin resistance as selectable marker. Transformation frequencies varied from 1.9 to 2.5 transformants μg−1 of DNA by the PEG method, and from 33 to 153 transformants μg−1 of DNA by the biolistic procedure.
Editor: Oxford, UK: Blackwell Publishing Ltd
Idioma: Inglês

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High frequency gene transfer by microprojectile bombardment of intact conidia from the entomopathogenic fungus Paecilomyces fumosoroseus

Chaves Barreto, Cristine ; Cardoso Alves, Luciano ; Lima Aragão, Francisco José ; Rech, Elıćbio ; Schrank, Augusto ; Henning Vainstein, Marilene

Oxford, UK: Blackwell Publishing Ltd

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