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Comparison of Five Radioimmunoassay Systems for PAG Measurement: Ability to Detect Early Pregnancy in Cows

Ayad, A ; Sousa, NM ; Sulon, J ; Iguer-Ouada, M ; Beckers, JF

Reproduction in domestic animals, 2007-08, Vol.42 (4), p.433-440 [Periódico revisado por pares]

Oxford, UK: Oxford, UK : Blackwell Publishing Ltd

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Título:
Comparison of Five Radioimmunoassay Systems for PAG Measurement: Ability to Detect Early Pregnancy in Cows
Autor: Ayad, A ; Sousa, NM ; Sulon, J ; Iguer-Ouada, M ; Beckers, JF
Assuntos: Animal reproduction ; Animals ; Artificial insemination ; Biological and medical sciences ; Case-Control Studies ; Cattle ; Comparative studies ; cows ; Diagnostic tests ; Female ; Fundamental and applied biological sciences. Psychology ; Hormones ; Life sciences ; Mammalian reproduction. General aspects ; Measurement techniques ; Médecine vétérinaire & santé animale ; Predictive Value of Tests ; Pregnancy ; Pregnancy Proteins - blood ; Pregnancy, Animal - physiology ; radioimmunoassay ; Radioimmunoassay - veterinary ; Sciences du vivant ; Sensitivity and Specificity ; Vertebrates: reproduction ; Veterinary medicine & animal health
É parte de: Reproduction in domestic animals, 2007-08, Vol.42 (4), p.433-440
Notas: http://dx.doi.org/10.1111/j.1439-0531.2006.00804.x
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content type line 23
scopus-id:2-s2.0-34447272653
Descrição: This study was conducted to describe the minimum detection limit, reproducibility, accuracy, specificity and parallelism of different pregnancy-associated glycoprotein radioimmunoassay (PAG-RIA) systems: RIA-497, RIA-706, RIA-780, RIA-809 and RIA-Pool. Their ability to distinguish between non-pregnant and pregnant females at day 30 after artificial insemination (AI) was investigated. The antisera were raised in rabbits against different PAG preparations. All RIA systems proved to be sensitive, repeatable and accurate for measuring PAG concentrations. The dilutions of plasma samples taken at an early stage of pregnancy were found to be parallel to the standard curves. No cross-reaction was observed with different carbohydrates, either with Pregnant Mare Serum Gonadotropin (PMSG) or human Chorionic Gonadotropin (hCG). The concentrations of PAG in pregnant females at day 30 after AI were shown to be higher with the use of antisera R#706, R#780, R#809 and Pool when compared with antiserum R#497. All the RIA systems gave 100% sensitivity and negative predictive values. On the other hand, the use of antisera R#780 and R#809 resulted in lower specificity and positive predictive values. The present study clearly shows that the ability of PAG-RIA systems to diagnose pregnancy specifically at day 30 after AI can be improved by using a combination of antisera raised against different forms of PAG.
Editor: Oxford, UK: Oxford, UK : Blackwell Publishing Ltd
Idioma: Inglês

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Comparison of Five Radioimmunoassay Systems for PAG Measurement: Ability to Detect Early Pregnancy in Cows

Ayad, A ; Sousa, NM ; Sulon, J ; Iguer-Ouada, M ; Beckers, JF

Oxford, UK: Oxford, UK : Blackwell Publishing Ltd

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