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Evaluation of a novel microfluidic immuno-magnetic agglutination assay method for detection of dengue virus NS1 antigen

Alejo-Cancho, Izaskun ; Navero-Castillejos, Jessica ; Peiró Mestres, Aida ; Albarracín, Rosa ; Barrachina, Josep ; Navarro, Alexander ; Gonzalo, Verónica ; Pastor, Víctor ; Muñoz Gutiérrez, José ; Martínez, Miguel J

Public Library of Science (PLoS) 2020-02

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  • Título:
    Evaluation of a novel microfluidic immuno-magnetic agglutination assay method for detection of dengue virus NS1 antigen
  • Autor: Alejo-Cancho, Izaskun ; Navero-Castillejos, Jessica ; Peiró Mestres, Aida ; Albarracín, Rosa ; Barrachina, Josep ; Navarro, Alexander ; Gonzalo, Verónica ; Pastor, Víctor ; Muñoz Gutiérrez, José ; Martínez, Miguel J
  • Assuntos: Dengue ; Infeccions ; Infections ; Nanoparticles ; Nanopartícules
  • Notas: Articles publicats en revistes (Medicina)
    PLoS Neglected Tropical Diseases, 2020, vol. 14, num. 2, p. e0008082
    https://doi.org/10.1371/journal.pntd.0008082
    http://hdl.handle.net/2445/170205
    Reproducció del document publicat a: https://doi.org/10.1371/journal.pntd.0008082
    1935-2735
    699638
    32069280
  • Descrição: BACKGROUND: Dengue virus (DENV) is the most important arbovirus worldwide, causing infections in endemic countries and returning travellers from these areas. Rapid diagnostic tests are needed to improve patient management and monitor local transmission. The detection of DENV non-structural protein 1 (NS1) is a useful tool for the diagnosis, but the currently available methods can be time consuming or lack sensitivity. The objective of our study was to evaluate a new rapid and semi-quantitative microfluidic DENV NS1 immuno-magnetic agglutination assay based on aggregation of magnetic nanoparticles detected by an electronic reader (Virotrack Dengue Acute and Blubox, Blusense diagnostics, Copenhagen, Denmark). METHODOLOGY/PRINCIPAL FINDINGS: A panel of 135 serum samples from travelers returning from dengue endemic countries was analyzed (74 DENV positive samples including the four DENV serotypes, 26 Zika virus positive samples, 25 chikungunya virus positive samples, 5 malaria positive samples and 5 negative samples). Samples were tested by three different antigen detection methods: SD Dengue NS1 Ag ELISA, SD BIOLINE Dengue Duo and ViroTrack Dengue Acute. The sensitivity observed for SD Dengue NS1 Ag ELISA, ViroTrack Dengue Acute and SD BIOLINE Dengue Duo was 97.2%, 91.1% and 68.1%, respectively. All methods showed high specificity (98.4% for ViroTrack Dengue Acute and 100% for both SD Dengue NS1 Ag ELISA and SD BIOLINE Dengue Duo). SD Dengue NS1 Ag ELISA and ViroTrack Dengue Acute only failed to detect samples positive for DENV-2. CONCLUSIONS/SIGNIFICANCE: ViroTrack Dengue Acute is a sensitive and specific assay for DENV NS1 detection. It provides faster results than the ELISA method and a better performance than the rapid immunochromatographic tests. ViroTrack Dengue Acute could represent a valuable tool for rapid diagnosis of DENV infections in returning travellers from endemic countries.
  • Editor: Public Library of Science (PLoS)
  • Data de criação/publicação: 2020-02
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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