skip to main content
Idioma:

Next-generation sequencing approach to investigate genome variability of Parapoxvirus in Canadian muskoxen (Ovibos moschatus)

Dalton, Chimone S. ; Workentine, Matthew L. ; Leclerc, Lisa-Marie ; Kutz, Susan ; van der Meer, Frank

Infection, genetics and evolution, 2023-04, Vol.109, p.105414-105414, Article 105414 [Periódico revisado por pares]

Netherlands: Elsevier B.V

Texto completo disponível

Citações Citado por
  • Título:
    Next-generation sequencing approach to investigate genome variability of Parapoxvirus in Canadian muskoxen (Ovibos moschatus)
  • Autor: Dalton, Chimone S. ; Workentine, Matthew L. ; Leclerc, Lisa-Marie ; Kutz, Susan ; van der Meer, Frank
  • Assuntos: Animals ; Arctic ; Canada ; Canada - epidemiology ; Communicable Diseases ; Genome ; Goats ; High-Throughput Nucleotide Sequencing ; Muskox ; NextSeq ; NGS ; Orf virus ; Orf virus - genetics ; Parapoxvirus ; Phylogeny ; Ruminants ; Sheep
  • É parte de: Infection, genetics and evolution, 2023-04, Vol.109, p.105414-105414, Article 105414
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: In 2016, the first orf virus, a double-stranded DNA (dsDNA) virus of the genus parapoxvirus, from a muskox was isolated on Victoria Island, Nunavut (NU), Canada. We used deep sequencing on DNA extracted from orf virus-positive tissues from wild muskoxen from locations on Victoria Island and the adjacent mainland. Orf virus sequence reads derived from four samples were nearly identical. The consensus sequences generated from pooled reads of MxOV comprises of a large contiguous sequence (contig) of 131,759 bp and a smaller right terminal contig of 3552 bp, containing all coding sequences identified as Parapoxvirus. Individual gene comparisons reveal that MxOV shares genetic characteristics with reference strains from both sheep and goat origin. Recombination analysis using Bootscan, MAXCHI, GENECONV, CHIMAERA, SISCAN, and RDP algorithms within the RDP4 software predicted recombination events in two virulence factors, and a large 3000 bp segment of the MxOV genome. Partial B2L nucleotide sequences from strains around the world and other North American isolates were compared to MxOV using MUSCLE alignments and RAxML phylogenetic trees. MxOV was identical to our previously characterized isolate, and shared similarity with orf virus isolated from sheep and goats. The phylogenetic grouping of partial B2L nucleotide sequences did not follow the sample geographic distribution. More full genomes of orf virus, or at least full B2L gene squences, in wildlife are needed especially in North America to better understand the epidemiology of the disease in muskoxen. •Deep sequencing of DNA extracted from orf virus-positive wild muskoxen from on Victoria Island, Nunavut, Canada.•Sequences generated from pooled reads of MxOV comprises all coding sequences identified as Parapoxvirus.•Gene comparisons reveal MxOV shares characteristics with reference strains from sheep and goat origin.•Recombination analysis predicted events in two virulence factors, and a large segment of the MxOV genome.
  • Editor: Netherlands: Elsevier B.V
  • Idioma: Inglês
Links
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.