Idioma:

High activation and skewed T cell differentiation are associated with low IL‐17A levels in a hu‐PBL‐NSG‐SGM3 mouse model of HIV infection

Perdomo‐Celis, F. ; Medina‐Moreno, S. ; Davis, H. ; Bryant, J. ; Taborda, N. A. ; Rugeles, M. T. ; Kottilil, S. ; Zapata, J. C.

Clinical and experimental immunology, 2020-05, Vol.200 (2), p.185-198 [Periódico revisado por pares]

England: Oxford University Press

Texto completo disponível

Citações Citado por

Enviar para

Título:
High activation and skewed T cell differentiation are associated with low IL‐17A levels in a hu‐PBL‐NSG‐SGM3 mouse model of HIV infection
Autor: Perdomo‐Celis, F. ; Medina‐Moreno, S. ; Davis, H. ; Bryant, J. ; Taborda, N. A. ; Rugeles, M. T. ; Kottilil, S. ; Zapata, J. C.
Assuntos: Animals ; Antiretroviral therapy ; CC chemokine receptors ; CCR7 protein ; CD38 ; CD38 antigen ; CD4 antigen ; CD4-CD8 Ratio ; CD45RA antigen ; CD8 antigen ; Cell activation ; Cell differentiation ; Cell Differentiation - immunology ; Disease Models, Animal ; Histocompatibility antigen HLA ; HIV ; HIV Infections - immunology ; HIV Infections - pathology ; HIV-1 - physiology ; HLA‐DR ; Human immunodeficiency virus ; Humans ; IL‐17A ; Interleukin-17 - immunology ; Leukocytes (granulocytic) ; Lymphocytes ; Lymphocytes T ; Lymphoid tissue ; Macrophages ; Mice ; Mice, Inbred NOD ; Mice, SCID ; NSG‐SGM3 ; Original ; PD‐1 ; Peripheral blood ; Plasma levels ; Replication ; Stem cell factor ; Stem cells ; T cell ; T-Lymphocytes - immunology ; T-Lymphocytes - pathology ; Transgenic mice ; Virus Replication - immunology
É parte de: Clinical and experimental immunology, 2020-05, Vol.200 (2), p.185-198
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Summary The humanized NOD/SCID/IL‐2 receptor γ‐chainnull (NSG) mouse model has been widely used for the study of HIV pathogenesis. Here, NSG mice with transgenic expression of human stem cell factor (SCF), granulocyte–macrophage colony‐stimulating factor (GM‐CSF) and interleukin (IL)‐3 (NSG‐SGM3) were injected with peripheral blood leukocytes (PBL mice) from two HIV‐infected (HIV+) patients who were under anti‐retroviral therapy (ART; referred as HIV+ mice) or one HIV‐seronegative healthy volunteer (HIV−). Such mice are either hu‐PBL‐NSG‐SGM3 HIV+ or HIV− mice, depending on the source of PBL. The kinetics of HIV replication and T cell responses following engraftment were evaluated in peripheral blood and secondary lymphoid tissues. High HIV replication and low CD4 : CD8 ratios were observed in HIV+ mice in the absence of anti‐retroviral therapy (ART). Consistent with high activation and skewed differentiation of T cells from the HIV‐infected donor, HIV+ mice exhibited a higher T cell co‐expression of human leukocyte antigen D‐related (HLA‐DR) and CD38 than HIV− mice, as well as a shifted differentiation to a CCR7−CD45RA+ terminal effector profile, even in the presence of ART. In addition, HIV replication and the activation/differentiation disturbances of T cells were associated with decreased plasma levels of IL‐17A. Thus, this hu‐PBL‐NSG‐SGM3 mouse model recapitulates some immune disturbances occurring in HIV‐infected patients, underlying its potential use for studying pathogenic events during this infection. hu‐PBL‐NSG‐SGM3 mouse model recapitulates some immune disturbances occurring in HIV‐infected patients
Editor: England: Oxford University Press
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Anterior Resultado 5 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

High activation and skewed T cell differentiation are associated with low IL‐17A levels in a hu‐PBL‐NSG‐SGM3 mouse model of HIV infection

Perdomo‐Celis, F. ; Medina‐Moreno, S. ; Davis, H. ; Bryant, J. ; Taborda, N. A. ; Rugeles, M. T. ; Kottilil, S. ; Zapata, J. C.

England: Oxford University Press

Buscando em bases de dados remotas. Favor aguardar.