Idioma:

Altered expression of noncanonical Wnt pathway genes in paediatric and adult adrenocortical tumours

Mermejo, Livia M. ; Leal, Leticia F. ; Colli, Leandro M. ; Fragoso, Maria C.B.V. ; Latronico, Ana C. ; Tone, Luiz G. ; Scrideli, Carlos A. ; Tucci, Silvio ; Martinelli, Carlos E. ; Yunes, Jose A. ; Mastellaro, Maria J. ; Seidinger, Ana L. ; Brandalise, Silvia R. ; Moreira, Ayrton C. ; Ramalho, Leandra N. ; Antonini, Sonir R. ; Castro, Margaret

Clinical endocrinology (Oxford), 2014-10, Vol.81 (4), p.503-510 [Periódico revisado por pares]

Oxford: Blackwell Publishing Ltd

Texto completo disponível

Citações Citado por

Enviar para

Título:
Altered expression of noncanonical Wnt pathway genes in paediatric and adult adrenocortical tumours
Autor: Mermejo, Livia M. ; Leal, Leticia F. ; Colli, Leandro M. ; Fragoso, Maria C.B.V. ; Latronico, Ana C. ; Tone, Luiz G. ; Scrideli, Carlos A. ; Tucci, Silvio ; Martinelli, Carlos E. ; Yunes, Jose A. ; Mastellaro, Maria J. ; Seidinger, Ana L. ; Brandalise, Silvia R. ; Moreira, Ayrton C. ; Ramalho, Leandra N. ; Antonini, Sonir R. ; Castro, Margaret
Assuntos: Adolescent ; Adrenal Cortex Neoplasms - genetics ; Adrenal Cortex Neoplasms - metabolism ; Adult ; Aged ; beta Catenin - genetics ; beta Catenin - metabolism ; Biological and medical sciences ; Child ; Child, Preschool ; Endocrinopathies ; Female ; Fundamental and applied biological sciences. Psychology ; Gene expression ; General aspects ; Humans ; Immunohistochemistry ; Infant ; Male ; Medical sciences ; Middle Aged ; Mortality ; Mutation ; Tumor Suppressor Protein p53 - genetics ; Tumor Suppressor Protein p53 - metabolism ; Tumors ; Vertebrates: endocrinology ; Wnt Signaling Pathway - genetics ; Wnt Signaling Pathway - physiology ; Young Adult
É parte de: Clinical endocrinology (Oxford), 2014-10, Vol.81 (4), p.503-510
Notas:
istex:0E1EDF45474BB8538FA3E706999A505B33F16FF0
ark:/67375/WNG-FBQB8H92-V
Fapesp - No. 2007/58365-3; No. 2008/09276-0
ArticleID:CEN12462
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Summary Context The role of planar cell polarity (Wnt/PCP) and calcium‐dependent (Wnt/Ca) noncanonical Wnt pathways in adrenocortical tumours (ACTs) is unknown. Objectives To investigate the gene expression of Wnt/PCP and Wnt/Ca pathways and its association with TP53 p.R337H and CTNNB1 mutations in paediatric and adult ACTs and to correlate these findings with clinical outcome. Patients Expression of noncanonical Wnt‐related genes was evaluated in 91 ACTs (66 children and 25 adults) by qPCR and the expression of beta‐catenin, P53 and protein effectors of Wnt/Ca (NFAT) and Wnt/PCP (JNK) by immunohistochemistry. TP53 and CTNNB1 genes were sequenced. Results TP53 p.R337H mutation frequency was higher in children (86% vs 28%), while CTNNB1 mutation was higher in adults (32% vs 6%). Mortality was higher in adults harbouring TP53 p.R337H and in children with CTNNB1 mutations. Overexpression of WNT5A, Wnt/Ca ligand, was observed in children and adults. Overexpression of MAPK8 and underexpression of PRICKLE, Wnt/PCP mediators, were observed in paediatric but not in adult cases. Cytoplasmic/nuclear beta‐catenin and P53 accumulation was observed in the majority of paediatric and adult ACTs as well as NFAT and JNK. Overexpression of MAPK8 and underexpression of PRICKLE were associated with mortality in children, while overexpression of WNT5A and underexpression of PRICKLE were associated with mortality in adults. Conclusions In our study, TP53 p.R337H and CTNNB1 mutations correlated with poor prognosis in adults and children, respectively. We demonstrate, for the first time, the activation of Wnt/PCP and Wnt/Ca noncanonical pathway genes, and their association with poor outcome in children and adults, suggesting their putative involvement in ACTs aggressiveness.
Editor: Oxford: Blackwell Publishing Ltd
Idioma: Inglês

Links

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Altered expression of noncanonical Wnt pathway genes in paediatric and adult adrenocortical tumours

Oxford: Blackwell Publishing Ltd

Buscando em bases de dados remotas. Favor aguardar.