Idioma:

Limited antimicrobial efficacy of oral care antiseptics in microcosm biofilms and phenotypic adaptation of bacteria upon repeated exposure

Schwarz, Sophia R. ; Hirsch, Stefanie ; Hiergeist, Andreas ; Kirschneck, Christian ; Muehler, Denise ; Hiller, Karl-Anton ; Maisch, Tim ; Al-Ahmad, Ali ; Gessner, André ; Buchalla, Wolfgang ; Cieplik, Fabian

Clinical oral investigations, 2021-05, Vol.25 (5), p.2939-2950 [Periódico revisado por pares]

Berlin/Heidelberg: Springer Berlin Heidelberg

Texto completo disponível

Citações Citado por

Enviar para

Título:
Limited antimicrobial efficacy of oral care antiseptics in microcosm biofilms and phenotypic adaptation of bacteria upon repeated exposure
Autor: Schwarz, Sophia R. ; Hirsch, Stefanie ; Hiergeist, Andreas ; Kirschneck, Christian ; Muehler, Denise ; Hiller, Karl-Anton ; Maisch, Tim ; Al-Ahmad, Ali ; Gessner, André ; Buchalla, Wolfgang ; Cieplik, Fabian
Assuntos: Adaptation ; Analysis ; Anti-Infective Agents ; Anti-Infective Agents, Local - pharmacology ; Antimicrobial agents ; Antiseptics ; Bacteria ; Bacterial artificial chromosomes ; Biofilms ; Chlorhexidine - pharmacology ; Dental caries ; Dentistry ; Drug resistance in microorganisms ; E coli ; Escherichia coli ; Gel electrophoresis ; Gingivitis ; Medicine ; Mimicry ; Minimum inhibitory concentration ; Original ; Original Article ; Protein expression ; RNA ; RNA, Ribosomal, 16S ; rRNA 16S ; Saliva ; Sodium lauryl sulfate ; Staphylococcus aureus ; Strains (organisms)
É parte de: Clinical oral investigations, 2021-05, Vol.25 (5), p.2939-2950
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Objectives The aims of this study were to investigate the antimicrobial efficacy of antiseptics in saliva-derived microcosm biofilms, and to examine phenotypic adaption of bacteria upon repeated exposure to sub-inhibitory antiseptic concentrations. Methods Saliva-derived biofilms were formed mimicking caries- or gingivitis-associated conditions, respectively. Microbial compositions were analyzed by semiconductor-based 16S rRNA sequencing. Biofilms were treated with CHX, CPC, BAC, ALX, and DQC for 1 or 10 min, and colony forming units (CFU) were evaluated. Phenotypic adaptation of six selected bacterial reference strains toward CHX, CPC, and BAC was assessed by measuring minimum inhibitory concentrations (MICs) over 10 passages of sub-inhibitory exposure. Protein expression profiles were investigated by SDS-PAGE. Results Both biofilms showed outgrowth of streptococci and Veillonella spp., while gingivitis biofilms also showed increased relative abundances of Actinomyces , Granulicatella , and Gemella spp. Antiseptic treatment for 1 min led to no relevant CFU-reductions despite for CPC. When treated for 10 min, CPC was most effective followed by BAC, ALX, CHX, and DQC. Stable adaptations with up to fourfold MIC increases were found in E. coli toward all tested antiseptics, in E. faecalis toward CHX and BAC, and in S. aureus toward CPC. Adapted E. coli strains showed different protein expression as compared with the wildtype strain. Conclusion Antiseptics showed limited antimicrobial efficacy toward mature biofilms when applied for clinically relevant treatment periods. Bacteria showed phenotypic adaptation upon repeated sub-inhibitory exposure. Clinical relevance Clinicians should be aware that wide-spread use of antiseptics may pose the risk of inducing resistances in oral bacteria.
Editor: Berlin/Heidelberg: Springer Berlin Heidelberg
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Limited antimicrobial efficacy of oral care antiseptics in microcosm biofilms and phenotypic adaptation of bacteria upon repeated exposure

Schwarz, Sophia R. ; Hirsch, Stefanie ; Hiergeist, Andreas ; Kirschneck, Christian ; Muehler, Denise ; Hiller, Karl-Anton ; Maisch, Tim ; Al-Ahmad, Ali ; Gessner, André ; Buchalla, Wolfgang ; Cieplik, Fabian

Berlin/Heidelberg: Springer Berlin Heidelberg

Buscando em bases de dados remotas. Favor aguardar.