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Molecular detection of prepatent Schistosoma mansoni infection in Biomphalaria glabrata snail vectors

Casotti, Márcia Oliveira ; Gryschek, Ronaldo Cesar Borges ; Paula, Fabiana Martins de ; Gomes-Gouvêa, Michele ; Pinho, João Renato Rebello ; Tuan, Roseli ; Dias-Neto, Emmanuel ; Luna, Expedito José de Albuquerque ; Espírito-Santo, Maria Cristina Carvalho do

Revista do Instituto de Medicina Tropical de São Paulo, 2020-01, Vol.62, p.e17-4 [Periódico revisado por pares]

Brazil: Instituto de Medicina Tropical de Sao Paulo

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Título:
Molecular detection of prepatent Schistosoma mansoni infection in Biomphalaria glabrata snail vectors
Autor: Casotti, Márcia Oliveira ; Gryschek, Ronaldo Cesar Borges ; Paula, Fabiana Martins de ; Gomes-Gouvêa, Michele ; Pinho, João Renato Rebello ; Tuan, Roseli ; Dias-Neto, Emmanuel ; Luna, Expedito José de Albuquerque ; Espírito-Santo, Maria Cristina Carvalho do
Assuntos: Animals ; Biomphalaria - parasitology ; Biomphalaria glabrata ; Brazil ; Deoxyribonucleic acid ; Disease Vectors ; DNA ; DNA, Helminth - analysis ; DNA, Ribosomal - analysis ; Health surveillance ; Host-Parasite Interactions ; Mitochondrial DNA ; Molecular diagnosis ; Mollusks ; Original ; Parasites ; Polymerase Chain Reaction ; Public health ; Schistosoma mansoni ; Schistosoma mansoni - genetics ; Snail ; Surveillance ; Tropical diseases ; TROPICAL MEDICINE ; Vector ; Vectors (Biology)
É parte de: Revista do Instituto de Medicina Tropical de São Paulo, 2020-01, Vol.62, p.e17-4
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Conceived and designed the study: MCCES, MOC, EJAL, RT, MGG, RCBG; contributed with analyses/tools: FMP, JRRP; wrote the paper: MCCES, MOC. All authors have read and approved the manuscript.
AUTHORS’ CONTRIBUTIONS
CONFLICT OF INTERESTS: The authors have declared that there is no conflict of interests.
Descrição: Approximately 240 million people worldwide are infected by Schistosoma. In Brazil, one of the main intermediate hosts of this parasite is Biomphalaria glabrata snails. The early detection of larval stages in intermediate hosts is an important challenge to public health, but it also represents an opportunity as a new alternative to indicate earlier natural infections before cercariae differentiation and emergence. In this context, we demonstrated that PCR amplification of a 28S gene fragment from the parasite does demonstrate S. mansoni infection in snails 14 days post infection. This conventional polymerase chain reaction amplified clear bands and was able to detect parasitic infection in the intermediate host B. glabrata under experimental conditions. However, we reinforce that this approach requires deeper investigations and further comparisons to confirm its specificity and sensitivity in earlier time points after miracidia infection. This approach has relevant potential as an effective molecular-based strategy for the monitoring of schistosomiasis transmission.
Editor: Brazil: Instituto de Medicina Tropical de Sao Paulo
Idioma: Inglês;Português

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Molecular detection of prepatent Schistosoma mansoni infection in Biomphalaria glabrata snail vectors

Casotti, Márcia Oliveira ; Gryschek, Ronaldo Cesar Borges ; Paula, Fabiana Martins de ; Gomes-Gouvêa, Michele ; Pinho, João Renato Rebello ; Tuan, Roseli ; Dias-Neto, Emmanuel ; Luna, Expedito José de Albuquerque ; Espírito-Santo, Maria Cristina Carvalho do

Brazil: Instituto de Medicina Tropical de Sao Paulo

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