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A wheat WRKY transcription factor TaWRKY10 confers tolerance to multiple abiotic stresses in transgenic tobacco

Wang, Chen ; Deng, Pengyi ; Chen, Liulin ; Wang, Xiatian ; Ma, Hui ; Hu, Wei ; Yao, Ningcong ; Feng, Ying ; Chai, Ruihong ; Yang, Guangxiao ; He, Guangyuan Zhang, Xiaoyu

PloS one, 2013-06, Vol.8 (6), p.e65120 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
A wheat WRKY transcription factor TaWRKY10 confers tolerance to multiple abiotic stresses in transgenic tobacco
Autor: Wang, Chen ; Deng, Pengyi ; Chen, Liulin ; Wang, Xiatian ; Ma, Hui ; Hu, Wei ; Yao, Ningcong ; Feng, Ying ; Chai, Ruihong ; Yang, Guangxiao ; He, Guangyuan
Zhang, Xiaoyu
Assuntos: Abiotic factors ; Abiotic stress ; Adaptation, Physiological ; Agriculture ; Amino acids ; Animals ; Arabidopsis ; Barley ; Biology ; Biophysics ; Blotting, Southern ; Blotting, Western ; Cloning, Molecular ; Cold ; Cold Temperature ; Cold treatment ; DNA binding proteins ; Drought ; Droughts ; Education ; Gene expression ; Genes ; Genetic engineering ; Genetically modified plants ; Genomes ; Genomics ; Germination ; Germination - physiology ; Glycine max ; Hydrogen peroxide ; Hydrogen Peroxide - pharmacology ; Immunoenzyme Techniques ; Immunoglobulin G - immunology ; International cooperation ; Kinases ; Laboratories ; Life sciences ; Malonaldehyde ; Malondialdehyde ; Moisture content ; Nicotiana - genetics ; Nicotiana - growth & development ; Nicotiana - metabolism ; Nuclei ; Oxidants - pharmacology ; Oxygen ; Physiology ; Plant growth ; Plant Proteins - genetics ; Plant Proteins - immunology ; Plant Proteins - metabolism ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; Polyethylene ; Polyethylene glycol ; Proline ; Proteins ; Rabbits ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Real-Time Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; Salinity tolerance ; Salt Tolerance ; Salts ; Signal transduction ; Sodium chloride ; Sodium Chloride - pharmacology ; Stress ; Stress, Physiological ; Stresses ; Subcellular Fractions ; Sugar ; Tobacco ; Transcription factors ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcriptional Activation ; Transgenic plants ; Trends ; Triticum - genetics ; Triticum - metabolism ; Triticum aestivum ; Water content ; Wheat
É parte de: PloS one, 2013-06, Vol.8 (6), p.e65120
Notas: Competing Interests: The authors have declared that no competing interests exist.
Conceived and designed the experiments: CW WH GY GH. Performed the experiments: CW PD XW HM NY YF RC. Analyzed the data: CW LC PD. Contributed reagents/materials/analysis tools: WH. Wrote the paper: CW.
Descrição: WRKY transcription factors are reported to be involved in defense regulation, stress response and plant growth and development. However, the precise role of WRKY transcription factors in abiotic stress tolerance is not completely understood, especially in crops. In this study, we identified and cloned 10 WRKY genes from genome of wheat (Triticum aestivum L.). TaWRKY10, a gene induced by multiple stresses, was selected for further investigation. TaWRKY10 was upregulated by treatment with polyethylene glycol, NaCl, cold and H2O2. Result of Southern blot indicates that the wheat genome contains three copies of TaWRKY10. The TaWRKY10 protein is localized in the nucleus and functions as a transcriptional activator. Overexpression of TaWRKY10 in tobacco (Nicotiana tabacum L.) resulted in enhanced drought and salt stress tolerance, mainly demonstrated by the transgenic plants exhibiting of increased germination rate, root length, survival rate, and relative water content under these stress conditions. Further investigation showed that transgenic plants also retained higher proline and soluble sugar contents, and lower reactive oxygen species and malonaldehyde contents. Moreover, overexpression of the TaWRKY10 regulated the expression of a series of stress related genes. Taken together, our results indicate that TaWRKY10 functions as a positive factor under drought and salt stresses by regulating the osmotic balance, ROS scavenging and transcription of stress related genes.
Editor: United States: Public Library of Science
Idioma: Inglês

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A wheat WRKY transcription factor TaWRKY10 confers tolerance to multiple abiotic stresses in transgenic tobacco

Wang, Chen ; Deng, Pengyi ; Chen, Liulin ; Wang, Xiatian ; Ma, Hui ; Hu, Wei ; Yao, Ningcong ; Feng, Ying ; Chai, Ruihong ; Yang, Guangxiao ; He, Guangyuan Zhang, Xiaoyu

United States: Public Library of Science

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