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Functional characterization of iPSC-derived arterial- and venous-like endothelial cells

Rosa, S ; Praça, C ; Pitrez, P R ; Gouveia, P José ; Aranguren, X L ; Ricotti, L ; Ferreira, L Silva

Scientific reports, 2019-03, Vol.9 (1), p.3826-3826, Article 3826 [Periódico revisado por pares]

England: Nature Publishing Group

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  • Título:
    Functional characterization of iPSC-derived arterial- and venous-like endothelial cells
  • Autor: Rosa, S ; Praça, C ; Pitrez, P R ; Gouveia, P José ; Aranguren, X L ; Ricotti, L ; Ferreira, L Silva
  • Assuntos: Blood vessels ; Cell adhesion & migration ; Cell adhesion molecules ; E-selectin ; Endothelial cells ; Inflammation ; Intercellular adhesion molecule 1 ; Mechanical properties ; Nitric oxide ; Pluripotency ; Prostaglandin E2 ; Stem cells ; Thrombin ; Tumor necrosis factor-TNF ; Tumor necrosis factor-α ; Vascular cell adhesion molecule 1 ; Vascular endothelial growth factor ; Vasoactive agents
  • É parte de: Scientific reports, 2019-03, Vol.9 (1), p.3826-3826, Article 3826
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: The current work reports the functional characterization of human induced pluripotent stem cells (iPSCs)- arterial and venous-like endothelial cells (ECs), derived in chemically defined conditions, either in monoculture or seeded in a scaffold with mechanical properties similar to blood vessels. iPSC-derived arterial- and venous-like endothelial cells were obtained in two steps: differentiation of iPSCs into endothelial precursor cells (CD31 /KDR /VE-Cad /EphB2 /COUP-TF ) followed by their differentiation into arterial and venous-like ECs using a high and low vascular endothelial growth factor (VEGF) concentration. Cells were characterized at gene, protein and functional levels. Functionally, both arterial and venous-like iPSC-derived ECs responded to vasoactive agonists such as thrombin and prostaglandin E2 (PGE ), similar to somatic ECs; however, arterial-like iPSC-derived ECs produced higher nitric oxide (NO) and elongation to shear stress than venous-like iPSC-derived ECs. Both cells adhered, proliferated and prevented platelet activation when seeded in poly(caprolactone) scaffolds. Interestingly, both iPSC-derived ECs cultured in monoculture or in a scaffold showed a different inflammatory profile than somatic ECs. Although both somatic and iPSC-derived ECs responded to tumor necrosis factor-α (TNF-α) by an increase in the expression of intercellular adhesion molecule 1 (ICAM-1), only somatic ECs showed an upregulation in the expression of E-selectin or vascular cell adhesion molecule 1 (VCAM-1).
  • Editor: England: Nature Publishing Group
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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