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Detection of early response genes in rat adrenal stimulated by acth and FGF2 using infusion in situ system

Rozana Beatriz Franco Baccaro Claudimara Ferini Pacicco Lotfi; Congress of the Brazilian Society for Cell Biology (12. 2004 Campinas); Ibero-American Congress of Cell Biology (9. 2004 Campinas)

Abstracts Campinas, SP

Campinas 2004

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  • Título:
    Detection of early response genes in rat adrenal stimulated by acth and FGF2 using infusion in situ system
  • Autor: Rozana Beatriz Franco Baccaro
  • Claudimara Ferini Pacicco Lotfi; Congress of the Brazilian Society for Cell Biology (12. 2004 Campinas); Ibero-American Congress of Cell Biology (9. 2004 Campinas)
  • Assuntos: ANATOMIA; BIOLOGIA CELULAR -- CONGRESSOS -- CONFERÊNCIAS
  • É parte de: Abstracts Campinas, SP
  • Notas Locais: Disponível em CD-ROM
  • Descrição: In order to obtain further information on the trophic mechanism of action of ACTH (adrenocorticotropin) and FGF2 (fibroblast growth factor) in an architectural-preserved intact gland system, we used an in situ isolated rat adrenal infusion technique. We analyzed early response genes expression, fos, jun and myc families of proto-oncogenes related with G0- G1-S transition of cell cycle to insight the composition of complex AP-1. Sprague-Dawley male rats were anesthetized and the left adrenal gland was isolated with clamps and the abdominal aorta cannulated. The gland was provided with DMEM/heparin (30min) and then treated (2h) with 10- 7M ACTH(A) or/and 20ng/ml FGF2(F) using an infusion pump (200µl/min). The glands were fixed, embedded in paraffin, cut at 6µm and the protein expression was determined by immunohistochemistry. Without stimulation Fra-2 and c-Jun proteins were detected in medulla but not in the adrenal cortex of control rats. Glands treated with ACTH showed Fra-2 and c-Jun expression in ZF and ZR but not in ZG while FGF2 treatments showed c-Jun protein expression also in ZG. A+F combination showed increase in the c-Jun levels in ZR and medulla but down regulation in ZF. In conclusion ACTH and FGF2 are capable of differentially regulating c-Jun protein and this situation could result in change in the ratio of protein forming the AP-1 complex
  • Editor: Campinas
  • Data de criação/publicação: 2004
  • Formato: [s. p.].
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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